NaCl、光和温度胁迫对龙眼胚性愈伤组织GPX酶活性的影响

以龙眼胚性细胞系LC2为材料，初步研究了在NaCl、光和温度胁迫下龙眼胚性愈伤组织ＧPX酶活性的变化规律。试验结果表明：（1）与对照相比，在盐胁迫下短期内ＧPX酶活性增加，随胁迫时间的延长，低盐胁迫下ＧPX酶活性先上升后下降，而高盐胁迫下ＧPX酶活性则呈下降趋势；（2）白光、绿光处理下，随光强的不断加强，GPX活性均呈先上升后下降趋势，且在同样光强下，白光处理的GPX活性均高于绿光；（3）龙眼EC的GPX活性在低温（高温）处理下，与对照相比，随温度不断降低（升高），GPX活性呈先升高后降低趋势。以上结果均表明，在一定逆境胁迫下，细胞内GPX可以起到应答外界刺激的作用，且活性呈规律表达，是植物在逆境胁迫下防御ROS伤害的主要标志之一。

The Effects of NaCl, Light and Temperature on the Activities of GPX of the Embryogenic Callus in Longan

In this experiment the Line LC2 of longan embryogenic callus was used as the material for the study on the changing tendency of Glutathione peroxidase (GPX) activities in the embryogenic callus of longan under the stress of salt (NaCl), light, and temperature. The results showed that: (1) compared with the control, the short-time salt treatment enhanced the activity of GPX, and with the time of stress prolonged, the activities of GPX under low-salt stress treatment ascending first and then descending, while it showed the downtrend under high-salt stress treatment; (2) under the treatment of the white light and green light, with the light intensity increased, the activities of GPX ascending first and then descending, and when the light intensity was the same, the activities of GPX was higher under the treatment of the white light than the green light; (3) under the treatment of low-temperature (high-temperature), with the temperature decreased (increased), the activities of GPX in the embryogenic callus of longan ascending first and then descending compared with those of the control. The above results indicated that under a certain stress, GPX played a role in response to external stimulation, and had a regular expression, and it was one of the main indicators of ROS injury defense under stress.