四川生态区甘蓝型油菜自交种和自然种品质测试的比较研究

摘 要：本文对四川生态区861份甘蓝型油菜自交种和自然种粗脂肪和硫苷含量的NIRS法测试结果进行了相关和回归分析。结果表明以NIR法测试甘蓝型油菜自然种粗脂肪含量普遍较自交种高，自交种粗脂肪含量与自然种粗脂肪含量绝对值差异达1%显著水平，自交种与自然种硫苷含量差异不显著。说明在甘蓝型油菜育种中，对粗脂肪的选择应侧重考虑自然种含量，兼顾考虑自交种含量，对硫苷的选择则可同时考虑自然种和自交种含量。自交种和自然种粗脂肪和硫苷含量的NIR法测试结果间相关关系、线性回归方程均达1%显著水平，说明在已知自交种粗脂肪（ x1 ）、硫苷（ x2 ）含量测试结果的情况下，可分别通过回归方程 y1 = 16.844 + 0.614 x1，y2 = -0.620 + 1.017x2预测自然种粗脂肪（ y1）和硫苷（ y2）含量。

Analysis of quality test values in Brissica Napus selfing seeds and open pollinating seeds in Sichuan ecological region

OBJECTIVECompared the test values of rude fat and glucosinolates contents in Brissica Napus L.selfing seeds and open pollinating seeds in Sichuan ecological region.METHODSelfing seeds and open pollinating seeds from 861 Brassica nanpus L.materials in Sichuan ecological region were tested by near-infrared spectroscopy(NIRS ) and the test values of two type seeds were analyzed by SPSS.RESULTSThe rude fat test values of open pollinating seeds was higher than the values of selfing seeds and the difference of the test values from two type seeds was at 1 percent significant level but was not significant when glucosinolates contents were concerned.The correlation coefficients of rude fat and glucosinolates contents test values of the two type seeds were at 1 percent significant level;The relationships of the test values of rude fat and glucosinolates contents between two type seeds could be described with linear regressive formulas respectively as follows:y1=16.844+0.614 x1(y1:the values of rude fat of open pollinating seeds;x1:the values of rude fat of selfing seeds);y2=-0.620+1.017x2(y2:the values of glucosinolates of open pollinating seeds;x2:the values of glucosinolates of selfing seeds).CONCLUSIONIt indicated that in Brissica Napus breeding selection the test values of open pollinating seeds by NIRS should be pay more attention than the ones of selfing seeds, and the glucosinolates contents test values of selfing seeds and open pollinating seeds have same importance.