基于28S rDNA D2区序列的陕南凹缘菱纹叶蝉分子系统学与遗传多样性研究

为探讨陕南桑园凹缘菱纹叶蝉(Hishimonus sellatus Uhler)系统关系与遗传多样性,对陕南7个主要蚕桑产区共132头凹缘菱纹叶蝉的 28S rDNA基因D2区序列进行核苷酸多样性和遗传差异分析,同时研究单倍型之间的分子系统关系和网络进化图。结果表明,总群体共存在多态性位点63个,单倍型8个,优势单倍型H_1占单倍型总数的74.2%,遗传分化程度较低。分子方差分析显示:种群内变异占总组分的98.94%,差异不显著,且其基因流系数Nst和种群地理距离之间不存在相关性(R~2=0.019 7),说明各个地理种群之间存在渐渗杂交。

Molecular Phylogeny and Genetic Diversity of Hishimones sellatus (Hemiptera:Cicadellidae)Based on28S rDNA D2 Region Sequence of Samples from South Shaanxi

The nucleotide diversity and genetic difference were analyzed based on28S rDNA D2 sequence of 132 Hishimones sellatus Uhler individuals from 7 major sericulture-producing regions in South Shaanxi.And then, the molecular phylogenetic relationship among haplotypes of the population were studied.The results showed that a total of 63 polymorphic loci existed in the total population, and formed 8 haplotypes, which the dominant haplotype H_1 accounted for 74.2% of the total haplotype, with a lower genetic differentiation.The molecular variance analysis showed that the variation within population accounted for 98.94% of the total component, and the difference was not significant, and there was no correlation between the gene flow coefficient Nst and the population geographic distance(R=0.019 7).All of these indicated that there was an introgression hybridization between the various geographical populations, it would provid a reference for further study of the population evolution.