| 番茄花粉萌发液体培养基的筛选和组分优化 |
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| 引用本文： | 王富,李文丽,秦公伟,袁振新.番茄花粉萌发液体培养基的筛选和组分优化[J].北方园艺,2008(11). |
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| 作者姓名： | 王富 李文丽 秦公伟 袁振新 |
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| 作者单位： | 青岛农业大学,园艺学院,山东,青岛,266109;陕西理工学院,生物科学与工程学院,陕西,汉中,723000 |
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| 基金项目： | 山东省农业良种工程资助项目
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青岛市科技攻关资助项目
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| 摘 要： | 以番茄自交系的新鲜混合花粉为材料,采用L16(45)正交试验设计,对番茄花粉的萌发液体培养基进行了筛选和优化.结果表明:浓度配比120 g/L蔗糖,120 mg/L硼酸,4 mg/L赤霉素和0.5 mg/L硫胺素的液体培养基为最佳培养基;硼酸、赤霉素和硫胺素对花粉萌发有显著影响,而蔗糖对花粉萌发没有明显作用.
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| 关 键 词： | 番茄 花粉 液体培养基 |
Screening of the Optimal Liquid Culture Medium for Tomato Pollen Germination |
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| WANG Fu,LI Wen-li,QIN Gong-wei,YUAN Zhen-xin.Screening of the Optimal Liquid Culture Medium for Tomato Pollen Germination[J].Northern Horticulture,2008(11). |
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| Authors: | WANG Fu LI Wen-li QIN Gong-wei YUAN Zhen-xin |
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| Institution: | WANG Fu1,LI Wen-li1,QIN Gong-wei2,YUAN Zhen-xin1 (1.College of Horticulture,Qingdao Agricultural University,Qingdao,Sh,ong 266109,China,2.Biological Science , Engineering Department,Shanxi University of Technology,Hanzhong,Shanxi 723000,China) |
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| Abstract: | Orthogonal experimental design of L16(45) was adopted to screen the optimal liquid culture medium according to the pollen germination rate.The influence of cane sugar,boric acid,gibberellic acid to pollen germination was analyzed.The result showed that the optimal liquid culture medium was cane sugar 120 g/L,boric acid 120 mg/L,gibberellic acid 40 mg/L and torulin 0.5 mg/L.Gibberellic acid,torulin and boric acid had much more obvious influence than cane sugar on pollen germination. |
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| Keywords: | Tomato Pollen Liquid culture medium |
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