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用RFLP鉴定普通小麦—纤毛鹅观草二体附加系中外源染色体的同源转化性
引用本文:陶文静,刘金元,王秀娥,刘大钧,陈佩度. 用RFLP鉴定普通小麦—纤毛鹅观草二体附加系中外源染色体的同源转化性[J]. 作物学报, 1999, 25(6): 657-661
作者姓名:陶文静  刘金元  王秀娥  刘大钧  陈佩度
作者单位:南京农业大学农业部作物细胞遗传重点开放实验室,江苏南京210095
基金项目:国家自然科学基金,国家“863”计划资助课题
摘    要:用小麦族7个部分同源群的40个RFLP探针对小麦——纤毛鹅观草二体附加系进行分析,在证实了原有细胞学鉴定结果的基础上,又进一步提供了纤毛鹅观草染色体部分同源群的分子证据。即96K025,96K026中添加的一对纤毛鹅观草染色体B属于第2部分同源群;96K012, 96K013中添加的一对染色体E属于第5部分同源群。对以上株系的衍生株系

关 键 词:普通小麦  纤毛鹅观草  二体异附加系  RFLP  同源转化性  
收稿时间:1998-12-22
修稿时间:1999-05-11

Homoeology Identification of the Alien Chromosomes or Their Arms Involved in T. aestivum-R. ciliaris Disomic Addition Lines by RFLP Analysis
Tao Wenjing Liu Jinyuan Wang Xiu'e Liu Dajun Chen Peidu. Homoeology Identification of the Alien Chromosomes or Their Arms Involved in T. aestivum-R. ciliaris Disomic Addition Lines by RFLP Analysis[J]. Acta Agronomica Sinica, 1999, 25(6): 657-661
Authors:Tao Wenjing Liu Jinyuan Wang Xiu'e Liu Dajun Chen Peidu
Affiliation:Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095
Abstract:Roegneria ciliaris chromosomes or it's arms involved in Triticum aestivum-Roegneria. ciliaris addition lines were successfully identified by means of RFLP analysis with 40 probes. Results confirmed previous cytological identification and determined the homoeologous grouping of the R. ciliaris chromosomes involved in the addition lines. Lines 96K025 and 96K026 are proved to contain an intact pair of R. ciliaris chromosome B, being identified as belonging to homoeologous group-2. The R. c. chromosomes E added to disomic addition lines 96K012 and 96K013 belong to homoeologous group-5. Line 96K030 contains a pair of R. c. chromosome D, identified as also homoeologous to wheat group-2 chromosomes. A pair of chromosome B long arms added to ditelosomic addkion line 96K033 is homoeologous to short arms of wheat group-2, and the short arm of chromosome B belongs to homoeologous group-2 long arm correspondingly. The results proved RFLP analysis a powerful tool for identifying the alien addition lines and clarifying the homoeology of alien R. ciliaris chromosomes or arms involved.
Keywords:T. aestivum  R. ciliaris  Disomic addition line  RFLP analysis  Homoeology
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