| 响应曲面法在枯草芽孢杆菌优化培养中的应用 |
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| 引用本文： | 黄宇,孙宝盛,孙井梅,张海丰,齐庚申.响应曲面法在枯草芽孢杆菌优化培养中的应用[J].安徽农业科学,2007,35(3):634-635,646. |
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| 作者姓名： | 黄宇 孙宝盛 孙井梅 张海丰 齐庚申 |
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| 作者单位： | 天津大学环境科学与工程学院,天津,300072;天津大学环境科学与工程学院,天津,300072;天津大学环境科学与工程学院,天津,300072;天津大学环境科学与工程学院,天津,300072;天津大学环境科学与工程学院,天津,300072 |
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| 基金项目： | 天津市科技发展计划项目（043115111-5）. |
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| 摘 要： | 基于Plackett-Burman设计及结果,采用响应曲面法研究了影响枯草芽孢杆菌生长的关键因素和最佳水平.结果表明,当牛肉膏为7.3 g/L,蛋白胨为7.1 g/L,温度33.5 ℃,pH值6.1时,菌体生长量最大.进而分析2因素间交互作用的等高线图表明:牛肉膏5.7～8.0g/L,蛋白胨5.2～8.0 g/L,温度33～34 ℃,pH值5.4～6.8时,可得到较高的菌体量.
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| 关 键 词： | 枯草芽孢杆菌 响应曲面法 minitab软件 菌体量 |
| 文章编号： | 0517-6611（2007）03-00634-02 |
| 修稿时间： | 2006-10-20 |
Application of Response Surface Methodolgy in Optimal Bacillus subtilis Culture |
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| HUANG Yu et al.Application of Response Surface Methodolgy in Optimal Bacillus subtilis Culture[J].Journal of Anhui Agricultural Sciences,2007,35(3):634-635,646. |
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| Authors: | HUANG Yu |
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| Institution: | School of Environmental Science and Engineering, Tianjin University, Tianjin 300072 |
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| Abstract: | Based on the results of a Plackett-Burman design,the most important factors affecting bacteria growth of Bacillus Subtilis were further investigated with the response surface methodology.The results showed that beef extract 7.3 g/L,peptone 7.1 g/L,temperature 33.5 ℃,pH 6.1,bacteria growth mass reached the maximum.Contour plots of two-factor interaction were studied and some conclusions were obtained as follows: beef extract 5.7～8.0 g/L,peptone 5.2～8.0 g/L,temperature 33～35 ℃,pH 5.4～6.8 were best for more biomass growth. |
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| Keywords: | Bacillus Subtilis Response surface methodology Minitab software Bacteria growth |
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