| 满园香梨叶片不定芽的诱导 |
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| 引用本文： | 汤浩茹,刘翠琼,罗娅.满园香梨叶片不定芽的诱导[J].果树学报,2005,22(6):706-709. |
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| 作者姓名： | 汤浩茹 刘翠琼 罗娅 |
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| 作者单位： | 1. 四川农业大学林学园艺学院,四川,雅安,625014
2. 四川农业大学林学园艺学院,四川,雅安,625014;北京林业大学生物科学与生物技术研究生院,北京,100083 |
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| 基金项目： | 教育部“新世纪优秀人才支持计划”项目(NCET-04-0905)，高等学校全国优秀博士学位论文作者专项基金项目(200253)，农业部“948”项目(2004246)。 |
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| 摘 要： | 采用正交试验设计研究了不同基本培养基、TDZ、生长素种类及其浓度4个因素组合对满园香梨(Pyrusus鄄suriensisMaxim.cv.Manyuanxiang)离体叶片不定芽再生的影响。结果表明,基本培养基的种类是影响叶片不定芽再生最重要的因素,其次是生长素的种类。通过叶片不定芽再生频率和平均每叶再生不定芽数分析表明,NN69+TDZ3.0mg/L+IAA0.2mg/L为满园香梨叶片不定芽发生的最佳培养基组合,在该培养基组合上,满园香梨叶片再生率和平均再生芽数分别达到了74.8%和4.40。同一叶片的基部和顶端比中部更有利于不定芽的再生。
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| 关 键 词： | 梨 离体叶片 不定芽再生 |
| 文章编号： | 1009-9980(2005)06-706-03 |
| 修稿时间： | 2005年3月7日 |
Adventitious bud regeneration from in vitro leaves of Pyrus ussuriensis cv. Manyuanxiang |
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| TANG Hao-ru,LIU Cui-qiong,LUO Ya.Adventitious bud regeneration from in vitro leaves of Pyrus ussuriensis cv. Manyuanxiang[J].Journal of Fruit Science,2005,22(6):706-709. |
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| Authors: | TANG Hao-ru LIU Cui-qiong LUO Ya |
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| Institution: | TANG Hao-ru1,LIU Cui-qiong1,2,LUO Ya1 |
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| Abstract: | The basal media, TDZ, the types and concentrations of auxin were used to compare their effects on adventitious shoots induction from leaves of pear (Pyrus ussuriensis Maxim. cv. Manyuanxiang) by using the orthogonal experiment design of L16(45). The results showed that the basal media played a vital role in inducing the adventitious buds, followed by plant growth regulators through comparison of bud regeneration rates and buds per leaf regenerated. 74.8% regeneration rate and 4.40 buds per leaf were achieved on the medium of NN69 TDZ 3.0 mg/L IAA 0.2 mg/L. The basal and apical leaf sections were more effective than middle leaf sections in inducing adventitious buds. |
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| Keywords: | Pear(Pyrus ussuriensis Maxim ) Leaves in vitro Adventitious bud regeneration |
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