| 基于ISSR标记的伏山楂起源及分类地位研究 |
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| 引用本文： | 韩晓颖,梁英海,王亚军,李峰,郭太君,薛艳杰. 基于ISSR标记的伏山楂起源及分类地位研究[J]. 吉林农业大学学报, 2009, 31(2) |
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| 作者姓名： | 韩晓颖 梁英海 王亚军 李峰 郭太君 薛艳杰 |
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| 作者单位： | 吉林农业大学园艺学院,长春,130118;吉林省农业科学院果树研究所,长春,130033;敦化市农业技术推广中心,敦化,133700 |
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| 基金项目： | 吉林省科技发展计划项目 |
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| 摘 要： | 利用ISSR标记对59份山楂材料进行了遗传多样性分析.从51条引物中筛选出15条,用于山楂的ISSR扩增,共扩增出122条带,其中多态性条带113条,多态性为92.62%.根据ISSR扩增结果,利用DPS软件进行分析,通过UPGMA对59份材料进行聚类分析,认为伏山楂为山楂属的一个独立种,与山楂亲缘关系较近.同时,探讨了我国北方分布的山楂属植物的亲缘关系.
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| 关 键 词： | 伏山楂 ISSR 聚类分析 遗传多样性 |
Analysis of the Origin and Classification of C. brettschnederi by ISSR Markers |
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| HAN Xiao-ying,LIANG Ying-hai,WANG Ya-jun,LI Feng,GUO Tai-jun,XUE Yan-jie. Analysis of the Origin and Classification of C. brettschnederi by ISSR Markers[J]. Journal of Jilin Agricultural University, 2009, 31(2) |
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| Authors: | HAN Xiao-ying LIANG Ying-hai WANG Ya-jun LI Feng GUO Tai-jun XUE Yan-jie |
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| Affiliation: | HAN Xiao-ying1,LIANG Ying-hai2,WANG Ya-jun1,LI Feng2,GUO Tai-jun1,XUE Yan-jie31.College of Horticulture,Jilin Agricultural University,Changchun 130118,China,2.Orchid Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,3.Agro-Tech Extension Center of Dunhua City,Dunhua 133700 |
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| Abstract: | Fifty-nine hawthorn germplasm were used as materials for analyzing their genome polymorphism by ISSR markers.15 primers selected from 51 primers were used for ISSR amplification.A total of 122 bands were generated,of which 113 bands were polymorphic bands(the percentage of polymorphic band,PPB=92.62%).According to the results of ISSR amplification,which were analyzed to Jaccard similarity coefficient by DPS software,the genetic relationship of hawthorn accessions were analyzed and 59 hawthorn were classifie... |
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| Keywords: | ISSR |
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