| 刺梨四倍体诱导及其ISSR分析 |
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| 引用本文： | 王小平,雍洪俊,杜晋城,梁国鲁,李丛英,王明霞. 刺梨四倍体诱导及其ISSR分析[J]. 北方园艺, 2010, 0(17): 158-160 |
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| 作者姓名： | 王小平 雍洪俊 杜晋城 梁国鲁 李丛英 王明霞 |
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| 作者单位： | 南充市高坪区农业局,果树站,四川,南充,637100;西南大学,园艺园林学院,重庆,北碚,400716 |
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| 摘 要： | 以刺梨为试材,利用组织培养结合秋水仙素溶液浸泡法诱导刺梨多倍体,并对四倍体与二倍体进行ISSR分析。结果表明:刺梨诱导芽分化增殖的最适培养基为MS+6-BA0.5mg/L+IAA0.1mg/L,诱导率达到100%,增殖系数4.4以上。用0.2%的秋水仙素溶液处理48h诱导率最高,纯合体诱导率为24.5%。四倍体与二倍体ISSR结果显示扩增条带差异明显,表明诱导加倍过程中四倍体发生了基因组变异。
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| 关 键 词： | 刺梨 ISSR 四倍体 |
Tetraploid Induction and ISSR Analysis in Rosa roxburghii Tratt |
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| WANG Xiao-ping,YONG Hong-jun,DU Jin-cheng,LIANG Guo-lu,LI Cong-ying,WANG Ming-xia. Tetraploid Induction and ISSR Analysis in Rosa roxburghii Tratt[J]. Northern Horticulture, 2010, 0(17): 158-160 |
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| Authors: | WANG Xiao-ping YONG Hong-jun DU Jin-cheng LIANG Guo-lu LI Cong-ying WANG Ming-xia |
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| Affiliation: | WANG Xiao-ping1,YONG Hong-jun1,DU Jin-cheng1,LIANG Guo-lu2,LI Cong-ying1,WANG Ming-xia1 (1.Gaoping District Agriculture Bureau Fruit Stand of Nangchong City,Nanchong Sichuan 637100,2.College of Horticulture and Landscape,Southwest Agricultural University,Beibei Chongqing 400716) |
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| Abstract: | Using Rosa roxburghii Tratt.as the experimental material,carried on the culture in vitro and using the colchicin solution inducted polyploidy,and analysized genomic defferences of tetraploid and diploid by ISSR.The results indicated that the most suitable culture medium which the Roxburghii induction differentiation multiplies was MS+6-BA 0.5 mg/L+IAA 0.1mg/L,the inductivity had achieved 100%,the k-factor was above 4.4.And the highest inductivity with 0.2%colchicin solution treatment 48h,the pure synthesis ... |
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| Keywords: | Rosa roxburghii ISSR tetraploid |
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