利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能

 通过RNA干涉对一个水稻锌指蛋白基因OsZRL的功能进行分析。半定量RT PCR、定量PCR分析结果显示，转基因植株OsZRL基因表达水平显著下调。与野生型相比，OsZRL表达水平下调的转基因株系叶片变大，根系、茎秆更为发达，表明OsZRL的下调对水稻植株的生长有促进作用。OsZRL基因的表达模式和转基因幼苗表型显示OsZRL参与赤霉素、脱落酸信号途径。因而推测锌指蛋白OsZRL是受赤霉素、脱落酸调节的水稻生长发育负调控因子。

Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference

The function of a rice zinc finger protein gene (OsZRL) was investigated by RNA interference. The results of semi quantitative RT PCR and quantitative PCR analysis indicated that OsZRL expression levels were significantly downregulated in the transgenic plants. The OsZRL RNAi transgenic lines were characterized by bigger leaves, stronger roots and stems compared to wild  type plants, indicating that the suppression of OsZRL had a positive effect on rice growth. The expression pattern and transgenic seedlings phenotypes revealed that OsZRL was involved in gibberellin or abscisic acid signaling pathway. It suggests that zinc finger protein OsZRL might perform its function of negetive regulator in rice growth through the regulation of gibberellin and abscisic acid.