深绿木霉T95和球毛壳ND35混用对土传病害的防治效果

用深绿木霉T95和球毛壳ND35两种拮抗菌株以及两者的混合菌株对由瓜果腐霉Pa和立枯丝核菌Rs引起的猝倒病和立枯病的防治效果进行了初步研究。平皿对峙结果表明,对瓜果腐霉Pa抑制作用最大的为ND35+T95混合拮抗菌,生长抑制率为73.36%,高于拮抗菌ND35对瓜果腐霉Pa的生长抑制率47.87%。而对立枯丝核菌Rs的拮抗作用三者之间差异不显著。盆栽试验结果表明,在菜豆上ND35+T95混合菌株对猝倒病的防治效果高于单个菌株深绿木霉T95,但在黄瓜、番茄上差异不显著。田间试验结果表明,混合拮抗菌株ND35+T95组合对立枯病和猝倒病的防治效果分别为49.05%和60.37%,均高于单一拮抗菌株深绿木霉T95的防治效果,但与对照化学药剂恶霉灵相比存在一定的差异。

Control Efficacy of Soil-borne Plant Diseases with Mixtures of Antagonistic Trichoderma atroviride T95 and Chaetomium globosum ND35

Control efficacy against Pythium damping-off and Rhizoctonia rot have been preliminarily investigated with each single antagonistic fungal strains and their mixed fungal strains of C. globosum ND35 and T. atroviride T95.The results of dual culture on PDA plate showed the strongest inhibitions of Pa were the antagonist combination of ND35+T95 with 73.36%.respectively. Their inhibitions were higher than that of single strain of ND35. But inhibition of Rs by the combination ND35+T95 and single strain of T95 and ND35 were little difference.The results in Greenhouse indicated that biological control efficacy of Pythium damping-off of bean vegetables by mixtures of two antagonistic fungal strains were higher than that of single antagonistic fungal strains T. atroviride T95.The results of experiment in field demonstrated that the best control efficacy was the combination ND35+T95 with 49.05% and 60.37%. And biological control efficacy by mixtures of antagonistic fungal strains were higher than that of single antagonistic fungal strains T. atroviride T95. But there was some difference in compared with control of chemical fungicide.