正交试验法优化罗布麻叶中总黄酮的闪式提取工艺 |
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引用本文: | 白明,李连珍,王亚宁,王宪龄. 正交试验法优化罗布麻叶中总黄酮的闪式提取工艺[J]. 安徽农业科学, 2013, 0(18): 7761-7762,7767 |
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作者姓名: | 白明 李连珍 王亚宁 王宪龄 |
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作者单位: | 河南中医学院,河南郑州,450046;河南农业大学,河南郑州,450002 |
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基金项目: | 河南中医学院科技创新支持计划 |
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摘 要: | [目的]优化闪式提取器提取罗布麻叶中总黄酮成分的工艺条件。[方法]在单因素试验的基础上,运用L9(34)正交试验优选罗布麻叶总黄酮的闪式提取工艺。[结果]闪式提取法提取罗布麻叶中总黄酮的最佳工艺为:乙醇浓度55%,料液比1∶50(W/V,g/ml,下同),提取时间50 s,提取次数2次;在此条件下,最高提取率为4.24%。验证性试验用最佳工艺条件提取5次,RSD为1.07%。[结论]该工艺稳定可行,闪式提取高效、快速,不破坏成分,有推广价值。
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关 键 词: | 罗布麻叶(FOLIUM APOCYNI VENETI) 正交试验 闪式提取 总黄酮 |
The Optimization of Flash-type Extractional Process of Total Flavonoids in Apocynum Venetum Leaves by Orthogonal Experiment |
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Affiliation: | BAI Ming( 1.Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046;) |
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Abstract: | [Objective] To explore the best process of flash-type extractor for extracting total flavonoids in Apocynum venetum leaves.[Method] Using L9 (34) orthogonal experimental method to optimize the flash-type extractional process of total flavonoids in Apocynum venetum leaves by single factor and orthogonal experiment.[Result] The optimal extraction technology is:ethanol concentration 55%,ratio of solid to liquid 1:50,extraction time 50 s,2 times,the highest extraction rate is 4.24%.The optimum technique conditions for verification test is extracting for 5 times,RSD is 1.07%.[Conclusion] The process is stable and feasible,suitable for promotion,and the flash-type extraction is efficient,fast and no damage for the components. |
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Keywords: | Apocynum venetum leaves Orthogonal experiment Flash-type extraction Total flavonoids |
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