F4菌毛FaeG基因的克隆与序列分析

目的]克隆F4菌毛FaeG基因,并对其进行序列分析。方法]利用PCR方法从6株标准F4菌株中扩增FaeG基因片段,测序后用DNAStar软件进行分析和拼接,并与已发表的F4菌毛基因序列进行分析和比较,绘制基因进化树,比较它们之间的同源性和进化关系。结果]试验成功扩增出FaeG基因片段;所扩增的6株已知血清变异型F4大肠杆菌与标准F4菌株同属于各自基因型,且F4ab与F4ac间的进化关系比与F4ad菌株间的近。结论]通过该研究可推测F4菌株3个血清变异型的检测不仅可用单因子血清,而且可从DNA水平上进行检测。

Cloning and Sequence Analysis of Fimbial Subunit FaeG Gene in F4

Objective] The aim was to clone the fimbial subunit FaeG gene of F4 and analyze its sequence.Method] The FaeG gene fragments were amplified from 6 standard F4 strains by PCR method,and were analyzed and spliced by DNAStar after sequencing.The sequences were analyzed and compared with the published F4 fimbrial genes.The homology and phylogenetic relationship between them was analyzed by the phylogenetic tree.Result] The FaeG gene fragments were amplified successfully; the amplified 6 strains of known serum variant F4 E.coli and standard F4 strains belonged to their respective genotypes,and the evolutionary relationship between F4ab and F4ac was nearer than its between F4ab and F4ad strains.Conclusion] Through the research could infer that the detected of three serum variants of F4 strains not only could use single factor serum,but could be detected from the DNA level.