| 小黑杨抗虫基因的遗传转化 |
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| 引用本文： | 常玉广,刘桂丰,姜静,王玉成,王大海. 小黑杨抗虫基因的遗传转化[J]. 东北林业大学学报, 2004, 32(6): 30-31 |
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| 作者姓名： | 常玉广 刘桂丰 姜静 王玉成 王大海 |
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| 作者单位： | 东北林业大学,哈尔滨,150040;东北林业大学,哈尔滨,150040;东北林业大学,哈尔滨,150040;东北林业大学,哈尔滨,150040;东北林业大学,哈尔滨,150040 |
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| 摘 要： | 建立了小黑杨转基因受体系统，利用农杆茵介导法将蜘蛛神经毒肽与bt基因C末端肽组成的融合基因导入小黑杨中，获得了3个小黑杨转抗虫基因植株，转化子为A1、A2、A3，并对其进行了GUS活性及目的基因PCR检测。初步证明A1、A2、A3为转基因阳性植株。
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| 关 键 词： | 小黑杨 遗传转化 GUS PCR |
The Genetic Transformation of the Genes Resistant to Insect for Populus xiaohei |
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| Chang Yuguang,Liu Guifeng,Jiang Jing,Wang Yucheng,Wang Dahai. The Genetic Transformation of the Genes Resistant to Insect for Populus xiaohei[J]. Journal of Northeast Forestry University, 2004, 32(6): 30-31 |
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| Authors: | Chang Yuguang Liu Guifeng Jiang Jing Wang Yucheng Wang Dahai |
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| Abstract: | The acceptor system of tans-gene Populus xiaohei was established, and Populus xiaohei plants transformed with fusion gene which is composed of the C terminal of bt gene and the spider insecticidal peptide were gained through agrobacterium tumefaciens transformation. The transformed plants with insect-resistance were marked as A1, A2 and A3, and GUS activity and PCR detection were conducted. The results show that the fusion gene has been transformed into the plants. |
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| Keywords: | Populus xiaohei Genetic transformation GUS PCR |
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