水牛子宫内膜腺上皮细胞和基质细胞的分离·培养和生物学特性研究

1材料与方法1．1材料1．1．1取材。孕早期成年水牛子宫由南宁某屠宰场提供。1．1．2试剂。一次性细胞培养皿地幻自美同BD公司；新生牛血清购A杭州四季青公司；雌二醇购自宁波市激素制品有限公司；离糖型DMEM细胞培养液粉剂、DPBS粉剂和胶原酶I购自美因GIBCO公司；细胞角蛋白单抗和：二抗购白广州深达生物制品公司。另外，青霉素、链霉素、胰蛋门酶、表皮生氐因子（EGF）、

Isolation, Cuture and Biological Characteristic Analysis of Stromal and Glandular Epithelial Cells of Buffalo (Bubalus bubalis) Endometrium

Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.