海地瓜中具吸湿保湿特性的胶原蛋白肽提取工艺研究

本文研究了海地瓜（Acaudina molpadioides）胶原蛋白多肽的提取工艺。利用氯化钠溶液去除海地瓜中的非胶原蛋白，通过单因素实验得出去除非胶原蛋白氯化钠的最佳浓度和体积：浓度为9％，体积为10倍海地瓜质量的氯化钠溶液，浸泡24h，可除去的非胶原蛋白占总蛋白的11．5％。通过单酶实验，选择AS．1398中性蛋白酶为实验用酶。以吸湿性和保湿性为指标，在单因素水平的基础上，采用正交实验对制备海地瓜胶原蛋白多肽的条件进行了优化，获得最佳工艺条件为：料液比为1：2、温度40℃、加酶量2400U／g、pH7．5、时间3h，胶原蛋白多肽的吸湿率和保湿率分别为6．11％和81．23％。

Study on extracting collagen peptides with characters of moisture retention and absorption capacity from Acaudina molpadioides

The extraction technology of collagen peptides from acaudina molpadioides was studied in this article. The non - collagen protein of acaudina molpadioides was separated by sodium chloride solution, using single factor experiment to validate the optimal conditions of sodium chloride solution including the concentra- tion of 9%, 10 times volume of the quality of acaudina molpadioides, marinated for 24h. Under these terms, the proportion of separated non - collagen protein in the total protein accounted for 11. 5%. Based on single enzyme factor experiment, AS. 1398 neutral protease was selected to hydrolyze collagen protein of acaudina molpadioides. Considering the moisture -absorption and moisture -retention, orthogonal experiment was conducted to optimize the preparation of the collagen peptides from acaudina molpadioides, with the results of the solid/liquid ratio 1： 2, temperature 40℃, amount of added enzyme 2400 U/g, pH 7. 5, and reacting time 3h, the moisture absorbency and moisture -retention ability achieved to 6. 11% and 81. 23%, respectively.