体外药动模型中恩诺沙星对嗜水气单胞菌的药动及药效研究

为降低嗜水气单胞菌耐药菌株的产生速度,延长恩诺沙星在水产动物上的应用年限,通过建立体外模型的方法,研究恩诺沙星对嗜水气单胞菌的药动-药效(PK-PD)参数。在消除半衰期为100h的模型内,2MIC(最小抑菌浓度)的恩诺沙星对嗜水气单胞菌能够抑制6h,6h后细菌出现再生长;4、8和16MIC对嗜水气单胞菌能够起到持续的抑制作用。在消除半衰期为168h的模型内,2MIC的恩诺沙星对嗜水气单胞菌能够抑制6h,6h后细菌出现再生长;4、8和16MIC对嗜水气单胞菌能够起到持续的抑制作用。结果表明,当AUC0→24/MIC>75.24±13.24,Cmax/MIC>4.10±0.12时,恩诺沙星对嗜水气单胞菌能够起到持续的抑制作用。

Pharmacodynamic effect of enrofloxacin against Aeromonas hydrophila in vitro pharmacokinetic model

The purposes of this research is to decrease the occurrence speed of resistant Aeromonas hydrophila and prolong the time limit of enrofloxacin application on aquatic creature. By using in vitro pharmacokinetic model, PK-PD parameter of Aeromonas hydrophila against enrofloxacin was studied. In the in vitro model with elimination half-life of 100 h, Aeromonas hydrophila was inhibited for 6 h by 2 MIC enrofloxacin and was continuously inhibited in the entire experiment period of 24 h by 4, 8 and 16 MIC enrofloxacin. In the in vitro model with elimination half-life of 168 h, the inhibitation effect of 2 MIC enrofloxacin on Aeromonas hydrophila lasted for 6 h and the bacteria regrew after that. 4, 8 and 16 MIC enrofloxacin showed continously inhabitative action on Aeromonas hydrophila. It concluded that con-tinous inhabitation of Aeromonas hydrophila by enrofloxacin could be achieved at the condition of AUC_(0→24)/ MIC>75.24±13.24, C_(max)/MIC>4.10±0.12.