| 小鼠受精卵在体内外发育成桑椹胚的对比研究 |
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| 引用本文： | 赖双英,努尔江,菊林花,王耀明. 小鼠受精卵在体内外发育成桑椹胚的对比研究[J]. 内蒙古农业大学学报(自然科学版), 1999, 20(4): 85-88 |
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| 作者姓名： | 赖双英 努尔江 菊林花 王耀明 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018
2. 新疆农业大学畜牧学院 |
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| 基金项目： | 内蒙古自然基金!（120120） |
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| 摘 要： | 用１０单位孕马血清和１０单位人绒毛膜促性腺素使小白鼠超数排卵，自然交配后分两组，一组取受精卵，统计受精卵个数，并在ＣＺＢ培养基微滴内培养７８ｈ，统计发育成又桑椹胚的个数。另一组，让受精卵在体内自然发育７２ｈ，收集桑椹胚，统计其个数。比较研究受精卵在体内外发育到桑椹胚的能力。实验结果表明，每只鼠的平均冲出受精卵数为２８．２枚；受精诳在体外培养时桑椹胚的发育率为８８％；因此在体外培养时从每只鼠平均可得
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| 关 键 词： | 小鼠受精卵 体外发育 体内发育 |
STUDIES ON DEVDLOPIGN KM MOUSE FERTILIZED EGG IN VITRL AND IN VIVOINTO MORULA |
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| LAI Shuang-ying,Nuerjiang,JU Lin-hua,WANG Yao-ming. STUDIES ON DEVDLOPIGN KM MOUSE FERTILIZED EGG IN VITRL AND IN VIVOINTO MORULA[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 1999, 20(4): 85-88 |
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| Authors: | LAI Shuang-ying Nuerjiang JU Lin-hua WANG Yao-ming |
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| Abstract: | Developing KM mouse fertilized egg into morula was studied the mice were superovulated and the embryos were developed in vitro and in vivo from one cell embryo to morula and the number of them was counted.The results of the experiments showed that each mouse prodnced 28.2 fertilized eggs on average;When the embryos were cultured in CZB medium in vitro,the morula developing rate was 88.0%;which meant that an average of 25 morulae from each mouse can be got by culture in vitro; When the embryos were developed in vitro,an average of 7.2 morulae were produced from each mouse. |
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| Keywords: | mouse embryo in vitro in vivo |
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