| 稻瘟病菌dsRNA病毒筛选及其对寄主的影响 |
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| 引用本文： | 李斌,陈传远,钟杰,高必达. 稻瘟病菌dsRNA病毒筛选及其对寄主的影响[J]. 湖南农业大学学报(自然科学版), 2017, 43(2). DOI: 10.13331/j.cnki.jhau.2017.02.012 |
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| 作者姓名： | 李斌 陈传远 钟杰 高必达 |
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| 作者单位： | 湖南农业大学植物保护学院,湖南长沙 410128;植物病虫害生物学与防控湖南省重点实验室,湖南长沙 410128 |
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| 基金项目： | 公益性行业(农业)科研专项，湖南农业大学青年科学基金项目 |
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| 摘 要： | 应用纤维素吸附法对60个稻瘟病菌株进行真菌病毒筛选,发现14个菌株含有ds RNA病毒。对带毒菌株GD45通过利巴韦林–高温结合方法得到衍生脱毒菌株GD45–4,与带毒菌株相比,其菌丝更为饱满、菌丝较直,在PDA平板上菌落形态显得更为蓬松,颜色偏白。推测菌株GD45 ds RNA病毒消除后会对其表现型产生一定影响。
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| 关 键 词： | 稻瘟病菌 dsRNA 弱毒菌株 |
Screening of dsRNA viruses and their impacts on Magnaporthe oryzae |
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| LI Bin,CHEN Chuanyuan,ZHONG Jie,GAO Bida. Screening of dsRNA viruses and their impacts on Magnaporthe oryzae[J]. Journal of Hunan Agricultural University, 2017, 43(2). DOI: 10.13331/j.cnki.jhau.2017.02.012 |
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| Authors: | LI Bin CHEN Chuanyuan ZHONG Jie GAO Bida |
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| Abstract: | In this study, we screened the presence of mycoviruses from a collection of 60 Magnaporthe oryzae(M. oryzae) strains using the cellulose chromatography method and found that 14 strains harbored dsRNAs. A virus–curing derivative strain GD45–4 was obtained from the dsRNA–infecting strain GD45 by high temperature–ribavirin combined virus–curing methods. When compared to the original strain GD–45, strain GD45–4 showed a more full and straight hypha, and a whiter and fluffier colony morphology on PDA, indicating the elimination of dsRNAs affected the phenotype of strain GD45. |
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| Keywords: | Magnaporthe oryzae dsRNA hypovirulent strain |
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