甲基睾酮对雌斑马鱼内分泌干扰效应的研究

甲基睾酮(MT)广泛存在于造纸废水中,为了研究其对水生动物内分泌的干扰效应,选用斑马鱼为模式生物,开展甲基睾酮对斑马鱼的慢性毒性暴露实验。将雌性斑马鱼分别暴露在含10、50、100和150μg/L的甲基睾酮水环境中48 d,另设空白对照和0.01%甲醇对照,观察斑马鱼的个体生长指标及组织结构的变化。结果表明:当MT浓度为150μg/L时,斑马鱼全部死亡;而在0~100μg/L的MT暴露处理中,随着MT浓度的增加,斑马鱼的体重逐渐减轻,体长逐渐缩短,臀鳍逐渐变大,体色转向偏黄,出现了雄性化的特征;而且肝脏和性腺重量均明显减轻,卵巢出现明显的退化特征,肝细胞逐渐肿大,细胞核偏移中央,且伴随细胞中空等现象;其中,50和100μg/L MT处理的斑马鱼鳃丝变得高低不一,有的开始断裂甚至结构无法辨认。

Endocrine Disruption of Methyltestosterone on Zebra Fish

Methyltestosterone is widely present in papermaking wastewater drainage. In order to study endocrine disrupting effects it produces, this paper used zebra fish as model organism to conduct methyltestosterone zebra fish exposed to chronic toxicity experiments. The female Zebra fish were exposed to the water environment with 0, 10, 50, 100 and 150 μg/L methyl testosterone in 48 days and no MT group and 0.01% methanol group used as control group, to record their growth indicators changes and tissue damaged. The results showed that exposure to 150 μg/L MT Zebra fish all died. In other groups, with the growth of concentration, various indicators have been changed. MT has tiggered some symptoms to treated group zebra fish, such as oval cells degenerated; hepatocyte tumefaction, vacuolation and nuclear offseted. Besides that, in 50 and 100μg/L MT groups, fish fin filgaments showed uneven, some of them even broken or unrecognized.