| 利用SSR和SFP标记分析水稻叶色突变体的多态性 |
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| 引用本文： | 刘明浩,肖楠,方希林,陈茜霖,吴旺嫔,陈光辉,王悦.利用SSR和SFP标记分析水稻叶色突变体的多态性[J].湖南农业科学,2017(4). |
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| 作者姓名： | 刘明浩 肖楠 方希林 陈茜霖 吴旺嫔 陈光辉 王悦 |
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| 作者单位： | 1. 湖南农业大学农学院,湖南 长沙,410128;2. 湖南农业大学农学院,湖南 长沙 410128;南方粮油作物协同创新中心,湖南 长沙 410128 |
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| 基金项目： | 国家自然科学基金，湖南省教育厅优秀青年项目，湖南农业大学大学生创新性实验计划项目 |
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| 摘 要： | 利用1 286对SSR标记和66对SFP标记对水稻转绿型新叶黄化突变体ygr和正常绿叶水稻品种日本晴(NBP)的基因组进行多态性研究。结果表明:1 236对SSR标记和63对SFP标记有扩增产物,扩增效率为96.08%;亲本间共筛选到248对SSR多态标记和12对SFP多态标记,平均每条染色体有22对,多态检出率为19.23%;多态标记覆盖水稻基因组达1 626.2 c M,相邻多态标记间平均遗传距离为6.25 c M。不同类型标记间比较发现,SSR标记的多态检出率比SFP标记高1.1个百分点,两者相差不大。
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| 关 键 词： | 水稻 SSR标记 SFP标记 叶色 多态性 |
Polymorphism Analysis of Rice Leaf Color Mutant by SSR and SFP Markers |
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| LIU Ming-hao,XIAO Nan,FANG Xi-lin,CHEN Qian-lin,WU Wang-pin,CHEN Guang-hui,WANG Yue.Polymorphism Analysis of Rice Leaf Color Mutant by SSR and SFP Markers[J].Hunan Agricultural Sciences,2017(4). |
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| Authors: | LIU Ming-hao XIAO Nan FANG Xi-lin CHEN Qian-lin WU Wang-pin CHEN Guang-hui WANG Yue |
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| Abstract: | One thousand two hundred and eight six pairs of SSR markers and 66 pairs of SFP markers were used to find the genomic polymorphism between the new leaf yellowing mutant ygr and normal green leaf cultivar NBP. The results showed that 1236 pairs of SSR markers and 63 pairs of SFP markers had amplification products and amplification efficiency was 96.08%. A total of 248 pairs of SSR polymorphic markers and 12 pairs of SFP polymorphic markers were screened. There were 22 pairs of polymorphic markers on each chromosome, and the polymorphism was 19.23%. Polymorphic markers covered 1626.2 cM of the rice genome, and the average genetic distance was 6.25 cM between adjacent polymorphic markers. Compare by different types of markers, the polymorphism detection rate of SSR marker was higher than that of SFP marker by 1.1 percent point, with no significant difference. |
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| Keywords: | rice SSR markers SFP markers leaf color polymorphism |
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