| 鸡白痢沙门菌减毒候选疫苗株的环境应激评价及主要生物学特性测定 |
| |
| 引用本文： | 王艳琳,满成连,冯政,陈素娟,秦涛,彭大新. 鸡白痢沙门菌减毒候选疫苗株的环境应激评价及主要生物学特性测定[J]. 中国预防兽医学报, 2019, 0(6): 616-621 |
| |
| 作者姓名： | 王艳琳 满成连 冯政 陈素娟 秦涛 彭大新 |
| |
| 作者单位： | 扬州大学兽医学院江苏省动物重要疫病与人兽共患病防控协同创新中心/江苏省家禽疫病防控工程技术研究中心 |
| |
| 基金项目： | 国家自然科学基金项目(31572530);公益行业农业科研专项(20130344);江苏省重点研发计划项目(现代农业)(BE2018315);江苏高校优势学科建设工程 |
| |
| 摘 要： | 为评估鸡白痢沙门菌减毒候选疫苗株对环境应激及消毒剂的抵抗力,本研究对S6702△rfaG△csgA、S6702△rfaL△csgA、S6702△rfaG△csgA△ompR和S6702△rfaL△csgA△ompR等4种鸡白痢沙门菌双基因缺失株和三基因缺失株进行了生长曲线、生物被膜形成能力、药敏试验、环境应激试验及消毒剂的灭活效果的测定。结果显示,4株缺失株均丧失生物被膜形成能力并保留了与亲本株相似的药物敏感性。多基因缺失株对pH为4.0和8.0的培养条件较野生菌株和单基因缺失株更为敏感,呈现微耐酸和不耐碱的特性;所有菌株对54℃热处理和紫外线处理均敏感。消毒剂对实验菌株的杀灭效果显示,野生菌株和各基因缺失株均对0.01%的聚维酮碘溶液敏感,基因缺失株相比野生菌株过硫酸氢钾复合物更为敏感。因此,鸡白痢沙门菌减毒候选疫苗株对环境应激的抵抗力均有所降低,本研究为该菌后续候选疫苗株的进一步筛选奠定了基础。
|
| 关 键 词： | 鸡白痢 减毒疫苗 耐药性 环境应激 消毒剂 |
Evaluation of resporses of Salmonella enterica serovar pullorum attenuated vaccine candidate strains to environmental stresses and disinfectants |
| |
| WANG Yan-lin,MAN Cheng-lian,FENG Zheng,CHEN Su-juan,QIN Tao,PENG Da-xin. Evaluation of resporses of Salmonella enterica serovar pullorum attenuated vaccine candidate strains to environmental stresses and disinfectants[J]. Chinese Journal of Preventive Veterinary Medicine, 2019, 0(6): 616-621 |
| |
| Authors: | WANG Yan-lin MAN Cheng-lian FENG Zheng CHEN Su-juan QIN Tao PENG Da-xin |
| |
| Affiliation: | (College of Veterinary Medicine,Yangzhou University,Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses/Jiangsu Engineering Technology Research Center for the Prevention and Control ofProvincial Poultry Disease,Yangzhou 225000,China) |
| |
| Abstract: | WANG Yan-lin;MAN Cheng-lian;FENG Zheng;CHEN Su-juan;QIN Tao;PENG Da-xin(College of Veterinary Medicine,Yangzhou University,Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses/Jiangsu Engineering Technology Research Center for the Prevention and Control ofProvincial Poultry Disease,Yangzhou 225000,China) |
| |
| Keywords: | Salmonella pullorum attenuated vaccine antibiotic susceptibility environmental stress disinfectants |
| 本文献已被 CNKI 维普 等数据库收录！ |
