广西猪伪狂犬病的流行监测及防控工作重点

为调查猪伪狂犬病（PR）目前在广西的流行情况，于2006～2008年对广西13个市122个不同规模猪场进行流行病学调查，并对163份病料及61份血清进行检测。结果显示，163份病料经PCR检测出阳性病料6份，阳性率为3．7％；对6株广西PRV gE胞外基因进行克隆测序比较及遗传进化分析发现，广西PRV毒株变异不大，6株广西PRVgE基因与国内外其他毒株之间核苷酸序列同源性均很高，介于96．4％～100．0％之间，氨基酸同源性为93．6％～100．0％，亲缘关系较为密切，应为同一毒株遗传进化而来；61份血清经gE—ELISA检测出阳性血清7份，阳性率11．5％，表明部分猪场存在PRV隐眭感染。调查结果肯定了广西在PR防控工作中取得的成绩，提出做好免疫、加强监测、淘汰隐性感染猪、提高猪体免疫力、净化种猪群是今后PR防控工作的重点。

Epidemiological monitoring and emphasis on controlling porcine pseudorabies in Guangxi

In order to investigate the present epidemic status of porcine pseudorabies(PR) in Guangxi,about 122 different pig farms in 13 cities of Guangxi were selected for epidemiological investigation and 163 samples and 61 serum samples were detected by PCR.The results showed that 6 samples of 163 samples were confirmed positive and the positive rate was 3.7%.The gE genes of six PRV strains from Guangxi were amplified and sequenced,the genetic evolution analy-sis results indicated that the variance of PRV strain f...