| 转基因产品中抗除草剂基因的PCR检测 |
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| 引用本文： | 许文涛,黄昆仑,邓爱科,罗云波. 转基因产品中抗除草剂基因的PCR检测[J]. 农业生物技术学报, 2005, 13(5): 575-579 |
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| 作者姓名： | 许文涛 黄昆仑 邓爱科 罗云波 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | The State High Science and Technology Program （863） （No.2001AA212291） of China. |
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| 摘 要： | 抗除草剂作物占全球转基因作物的75％以上，并且这类作物还有不断增加的趋势。首次建立并优化了对4类转基因作物（大豆（Glycine max）、玉米（Zea mays）、棉花（Gossypium hirsutnm）和油菜（Brassica campestris var.oleifera））的5种外源抗除草剂基因（bar、pat、cp4-epspsI、cp4-epsps2和gox）的PCR检测方法，并在实践中应用。结果表明，本方法灵敏特异、结果准确可靠，可对目前批准的所有抗除草剂转基因产品进行定性检测。
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| 关 键 词： | 聚合酶链式反应 抗除草剂基因 转基因产品 |
| 收稿时间： | 2004-09-30 |
| 修稿时间： | 2005-01-05 |
PCR for Anti-herbicide Genes Detection in Genetically Modified Organisms |
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| XU Wen-Tao,HUANG Kun-Lun,DENG Ai-Ke,LUO Yun-Bo. PCR for Anti-herbicide Genes Detection in Genetically Modified Organisms[J]. Journal of Agricultural Biotechnology, 2005, 13(5): 575-579 |
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| Authors: | XU Wen-Tao HUANG Kun-Lun DENG Ai-Ke LUO Yun-Bo |
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| Affiliation: | College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083,China |
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| Abstract: | The GMO (genetically modified organisms) containing anti-herbicide genes account for more than 75% of all GMO and the proportion is increasing. The 5 kinds of the anti-herbicide genes (bar, pat, cp4-epspsl, cp4-epsps2 and gox) were examined by PCR in 4 kinds of genetically modified crops (soybean (Glycine max), maize (Zea mays), cotton (Gossypinm hirsutnm) and rape (Brassica campestris var. oleifera)). Results indicated that the method found was sensitive, specific and credible, and all genetically modified crops containing the anti-herbicide genes could be analyzed by this method. |
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| Keywords: | PCR anti-herbicide gene genetically modified organisms |
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