Isolation and transmission ofCowdria
ruminantium (causal agent of heartwater disease) in Blue Nile Province,Sudan |
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| Authors: | F Jongejan S P Morzaria Omer A Shariff Hashim M Aboalla |
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| Institution: | (1) Tick and Tick-borna Diseases Control Project, FAO, P.O. Box 1117, Khartoum, (Sudan);(2) Present address: UNDP/FAO Project ZAM/77/002, Animal Disease Control, P.O. Box 30563, Lusaka, (Zambia);(3) Present address: International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, (Kenya) |
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| Abstract: | Heartwater was diagnosed in sheep at the Umm Banein Livestock Research Station, Blue Nile Province, Sudan.Cowdria
ruminantium was isolated from sheep by subinoculation of blood into a goat. AdultAmblyomma
lepidum ticks fed as nymphs on this goat transmitted heartwater to a Friesian (Bos
taurus) calf. Brain stabilates from this calf, either snapfrozen into liquid nitrogen or frozen slowly in a deepfreezer at –70°C, with DMSD as cryoprotectant, were infective to cattle when inoculated subcutaneously. |
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