红叶石楠愈伤组织抗褐化研究以及悬浮细胞系的建立

以红叶石楠无菌苗叶片为试材,诱导愈伤组织并建立悬浮细胞系。结果表明:红叶石楠愈伤组织初代诱导最佳培养基为MS+6-BA 0.5 mg/L+2,4-D 1.0 mg/L+NAA 0.1 mg/L,诱导率可达97%。继代培养以MS+6-BA 1.0 mg/L+NAA 0.1 mg/L为宜。针对红叶石楠愈伤组织继代培养过程中易褐化的问题,通过正交试验探讨了不同吸附剂、抗氧化剂及其它因素对褐化的影响。采用MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+Vc 1.0 mg/L+Ch 100 mg/L附加1%蔗糖的继代培养基,可以明显降低红叶石楠愈伤组织的褐化现象。将胚性愈伤组织接种到1/2 MS+6-BA 1.0 mg/L+NAA 0.1 mg/L附加2%蔗糖,pH 5.5液体培养基中悬浮震荡培养获得了分散性好、色泽鲜艳的悬浮细胞。

Studies on the decreasing browning of callus and the establishment of suspension cell lines from Photinia frasery

Asepsis leaves of Photinia frasery were employed to study callus tissue culture and establishment of suspension cell lines.The results showed that the appropriate medium for callus induction is MS 6-BA 0.5 mg/L 2,4-D 1.0 mg/L NAA 0.1 mg/L;Inductivity is up to 97%;And the medium of multiplication is MS 6-BA 1.0 mg/L NAA 0.1 mg/L.Orthogonal tests were performed to find out the effects of different adsorbents,anti-oxidants and other factors on callus browning.The medium for anti-browning is MS 6-BA 1.0 mg/L NAA 0.2 mg/L VitC 1.0 mg/L Ch 100 mg/L with1% sugar.The calli obtained were subsequently cultured in liquid medium 1/2 MS 6-BA 1.0 mg/L NAA 0.1 mg/L with 2% sugar,pH 5.5 for establishing suspension cell lines.The cells present good shape,dispersivity and bright yellow color.