Development of a programmable piggyback syringe pump and four-times-a-day injection regimen for superovulation in non-lactating Holstein cows |
| |
| Authors: | Abdul Razaq IRSHAD Taihei SASAKI Tomoaki KUBO Naoyuki ODASHIMA Keiji KATANO Takeshi OSAWA Toru TAKAHASHI Yoshiaki IZAIKE |
| |
| Institution: | 1)United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan;2)Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan;3)Icomes Lab, Morioka 020-0857, Japan;4)Department of Veterinary Medicine, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan |
| |
| Abstract: | The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3– < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively). |
| |
| Keywords: | Cows Follicles Piggyback syringe pump Porcine follicle-stimulating hormone (pFSH) Superovulation |
|
|
