盐胁迫下表油菜素内酯对沟叶结缕草愈伤组织生长和再生的影响

表油菜素内酯(EBL)在促进植物生长发育及提高植物抗逆性方面发挥着重要作用。本研究以沟叶结缕草[Zoysia matrella(L.) Merr.]胚性愈伤组织为材料,采用不同浓度NaCl进行盐胁迫处理,并在不同盐胁迫下加入不同浓度的EBL,分析EBL对沟叶结缕草愈伤组织生长和再生,以及愈伤组织和再生植株叶片中过氧化氢酶(CAT)、过氧化物酶(POD)、超氧岐化酶(SOD)活性和丙二醛(MDA)含量的影响。结果表明,当NaCl浓度≥0.6%时,沟叶结缕草愈伤组织生长受到抑制;当NaCl浓度≥0.4%时,沟叶结缕草愈伤组织再生受到抑制。在盐胁迫下,加入0.05 mg·L^-1EBL对愈伤组织生长的促进效果最显著,加入0.02 mg·L^-1EBL对愈伤组织再生的促进效果最显著。当NaCl浓度为0.6%时,经0.05 mg·L^-1 EBL处理,沟叶结缕草愈伤组织的直径增长率升高51.74%,鲜重增长率升高49.73%;当NaCl浓度为0.4%时,经0.02 mg·L^-1EBL处理,愈伤再生成苗数提高38.1...

Effects of Epibrassinolide on Callus Growth and Regeneration of Zoysia matrella (L.) Merr. Under Salt Stress

Epibrassinolide (EBL) has been shown to play an important role in plant growth and stress resistance. In this study, the callus of Zoysia matrella (L.) Merr. was treated by salt stress with different NaCl concentrations, and different concentrations of EBL were added under the salt stress. The influences of EBL on callus growth and plant regeneration, the activites of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) of the callus and the leaves from the regenerated plantlets were measured and analyzed. The results showed that when the salt concentrations were equal or greater than 0.6%, the growth of callus was inhibited and the callus regeneration was inhibited when the salt concentrations were equal or greather than 0.4%. The effect of adding 0.05 mg·L^-1EBL was the most significant in improving the salt tolerance of callus, while 0.02 mg·L^-1EBL was the most significant to improve the salt tolerance of callus regeneration. When the NaCl concentration was 0.6%, the growth rate of diameter increased by 51.74%, as well as the fresh weight increased by 49.73% of callus under the treatment of 0.05 mg·L^-1EBL.When the NaCl concentration was 0.4%, the amount of plantlets increased by 38.14% and the roots with lengths equal or greater than 5 mm increased by 20.12% of regeneration under the treatment with 0.02 mg·L^-1EBL. In this study, plants of Zoysia matrella with stronger salt tolerance were obtained through experiments, which indicates that the addition of EBL in vitro can effectively alleviate salt stress and promote the growth and regeneration of Zoysia matrella callus. The results of this study also provide a basis for the wider application of Zoysia matrella in saline-alkali land improvement.