广东石豆兰不同溶剂提取物抗氧化及与总黄酮、总酚含量的关系

为明确广东石豆兰的抗氧化能力及抗氧化成分,以鳞茎为材料,以甲醇、乙醇、丙酮和乙酸乙酯为提取剂得到4种提取物,测定了4种提取物中总黄酮、总酚含量及其抗氧化活性,并分析了两者之间的关系。结果表明,石豆兰总酚含量高于总黄酮,乙醇为总酚和总黄酮2种物质的最佳提取溶剂;甲醇提取物对羟基自由基清除力和对Fe²⁺的螯合力最强,对DPPH自由基清除效果最差;丙酮提取物具有最强的总还原力和清除超氧阴离子的能力,而清除羟基自由基能力最弱;乙酸乙酯提取物清除DPPH自由基的能力最强,但总还原力、清除超氧阴离子的能力、Fe²⁺的螯合力最弱。此外,除了DPPH自由基外,其他4个抗氧化指标与总黄酮或总酚含量呈正相关(0.185

Antioxidant Activities and Their Relationship With Contents of Total Flavonoids and Total Phenols of Various Solvent Extracts From Bublophyllum kwangtungense

In order to clarify the antioxidant capacity and antioxidant components of B. kwangtungense, bulb extracts were obatained with different solvents(methanol, ethanol, acetone and ethylacetatefour),the antioxidant activities and total flavonoids, total phenols of four extracts were tested, and their correlation was investigated. Results showed that: The content of total phenol was higher than total flavonoids, and ethanol was the best solvent for two ingredients. Methanol extract had the highest scavenging ability to hydroxyl radical and chelation power to Fe²⁺, while the scavenging effect to DPPH free radical ability was the weakest. Total reducing power and scavenging ability to superoxide anions of acetone extract were the highest, and the scavenging ability to hydroxyl radical was the weakest. The scavenging to DPPH free radical ability of ethylacetate extract was the strongest, while it had the weakest reducing power, scavenging ability to superoxide anions and chelation ability to Fe²⁺. Except DPPH free radical, the other four antixidant indicators had postive correlations with total flavonoids or total phenols(0.185<r<0.969, and the correlation between total reducing power and total flavonoids was significant. These results provided a reference for the extraction, further development and utilization of the antioxidants in B. kwangtungense.