牦牛血低聚肽对小鼠抗疲劳能力和缺氧H9c2细胞的影响

为研究牦牛血低聚肽对缺氧所致H9c2心肌细胞损伤以及对运动后小鼠抗疲劳能力的影响,本研究通过建立H9c2 细胞缺氧损伤模型以及小鼠负重游泳试验,研究其抗缺氧能力以及抗疲劳能力。结果表明,与正常对照组相比,缺氧组的H9c2细胞存活率极显著降低(P<0.01),表明缺氧对H9c2心肌细胞有一定损伤;与缺氧组相比,低聚肽干预组的细胞存活率增加且存在剂量依赖性;与常用抗缺氧药物红景天苷相比,牦牛血低聚肽高剂量组细胞存活率为75.1%,而红景天苷组为88.0%。牦牛血低聚肽及其复合肽均能延长小鼠负重游泳时间,提高运动后小鼠肝脏肝糖元(HG)含量,降低运动后小鼠血清乳酸(BLA)和血清尿素氮(BUN)含量。综上,牦牛血低聚肽对缺氧介导的心肌细胞损伤具有一定的保护作用,能提高小鼠的抗疲劳能力,且牦牛血低聚肽与大豆低聚肽按照 5∶5 比例复配后的抗疲劳能力优于单一使用牦牛血低聚肽。本研究结果为耐缺氧活性因子的研究提供了一条新途径。

Effects of Yak Blood Oligopeptides on Anti-fatigue Ability of Mice and Hypoxia H9c2 Cells

In order to study the effect of yak blood oligopeptides on hypoxia-induced H9c2 cardiomyocyte injury and anti-fatigue ability of exercise mice, the anti-fatigue ability and anti-hypoxia ability of yak blood oligopeptides were preliminarily studied by hypoxia-induced injury model in H9c2 cells and weight-bearing swimming test in mice. The results showed that the cell viability of the hypoxic group was significantly lower than that of the normal control group (P<0.01), indicated that hypoxia caused damage to the H9c2 cardiomyocytes; at the same time, compared with the hypoxic group, the cell viability of the yak blood oligopeptides intervention group increased and there was a dose-dependent, compared with the commonly used anti-hypoxic drug salidroside. The survival rate of the high dose group was 75.1%, while that of the Rhodiola group was 88%. Yak blood oligopeptides and their complex peptides could increase the swimming time of mice, increased the content of HG in the liver of mice after exercise, and decreased the content of BLA and BUN in the serum of mice after exercise. The results showed that the yak blood oligopeptides had protective effect on hypoxia-induced cardiomyocyte injury, and could improve the anti-fatigue ability of mice. The anti-fatigue ability of the combination of the yak blood oligopeptides and soybean oligopeptides was better than that of the single yak blood oligopeptides.The research results provide a new way for the research of hypoxia-tolerant active factors.