水稻黑条矮缩病抗病品种的筛选及其抗病机制初探

为有效防治水稻黑条矮缩病的大面积发生,本研究通过室内大量饲养无毒灰飞虱,饲毒后对浙江省20个主栽水稻品种进行传毒,人工接种水稻黑条矮缩病毒(rice black-streaked dwarf virus,RBSDV)后移栽田间,观察病苗症状,测量株高,统计发病率并分析病毒含量。结果表明,籼稻品种深两优5814和Y两优302的抗性较强,粳稻品种淮5和秀水14的抗性较弱。此外,病毒侵染后,籼稻品种深两优5814和Y两优302的OsMYC2和OsNPR1的mRNA表达水平显著高于粳稻品种淮5和秀水14。上述结果表明,籼稻品种深两优5814和Y两优302抗病毒能力与其体内茉莉酸(JA)和水杨酸(SA)激素水平相关,这为今后筛选抗RBSDV材料提供了新的策略和思路。

Screening of Rice Varieties and Preliminary Study on the Mechanism of Rice Black-Streaked Dwarf Virus Disease Resistance

To prevent and control Rice black-streaked dwarf virus (RBSDV) disease effectively, we reared a mass of small brown planthopper(Laodelphax striatellus, SBPH) with RBSDV-infected rice plants in the laboratory, then selected and analyzed the sensitivity of 20 local rice varieties in Zhejiang province to RBSDV infection by observing symptoms, measuring plant height and counting incidence after artificial inoculation assay. The results showed that the expression of RBSDV RNA decreased dramatically in indica rice, such as Shenliangyou 5814 and Y-liangyou 302, compared with that in japonica rice. Moreover, the expression of OsMYC2 and OsNPR1 in RBSDV-infected indica rice were significantly up-regulated than that in mocked indica plants. These results indicated that the resistance of the indica rice to RBSDV infection might be partly due to activate the jasmonic acid and salicylic acid pathway. Together, these results provided a new strategy for screening rice varieties resistance to RBSDV.