离子液体[C3mim]BF4对小白菜幼苗抗氧化特性的影响

为探讨离子液体1-丙基-3-甲基咪唑四氟硼酸(C₃mim]BF₄)对植物种子萌发、幼苗生长及生理生化的影响,采用水培法检测不同浓度(0、100、200、300、400、500 mg·L^-1)C₃mim]BF₄条件下小白菜幼苗的生长状况、活性氧水平、抗氧化酶活性及相关抗氧化酶基因表达。结果表明,浓度高于400 mg·L^-1的C₃mim]BF₄显著抑制种子萌发;500 mg·L^-1 C₃mim]BF₄显著抑制幼苗生长。C₃mim]BF₄处理使小白菜叶片活性氧$\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$、H₂O₂)水平及丙二醛(MDA)、脯氨酸(Pro)含量升高,且随着C₃mim]BF₄浓度的增加均呈逐渐升高的趋势。C₃mim]BF₄使小白菜叶片谷胱甘肽还原酶(GR)活性显著升高;浓度≤300 mg·L^-1的C₃mim]BF₄对过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)生成有一定的促进作用,而浓度高于400 mg·L^-1 C₃mim]BF₄处理显著抑制小白菜 APX活性,高于500 mg·L^-1 C₃mim]BF₄处理显著抑制小白菜 CAT活性;100~500 mg·L^-1C₃mim]BF₄处理显著抑制小白菜叶片超氧化物歧化酶(SOD)活性,且各处理组的SOD活性均显著低于对照。400 mg·L^-1 C₃mim]BF₄处理0~3 h,小白菜Gu/Zn-SOD和APX基因表达量升高,处理13 d时其SOD和APX基因表达量均显著低于对照;处理0~12 h时 CAT基因表达上调,处理24 h处理后CAT表达下调。本研究结果为揭示咪唑类离子液体毒性机理提供了理论依据。

Effects of the Ionic Liquid [C3mim]BF4 on Antioxidant Properties of Pakchoi Seedlings

To investigate effect of ionic liquid 1-propyl-3-methylimidazolium tetrafluoroborate (C₃mim]BF₄) on seed germination, seedling growth and physiological and biochemical characteristics of plants, hydroponic method was used to detect the changes of growth, level of active oxygen, activities of antioxidant system and antioxidant enzymes gene expression in pakchoi seedlings with different concentrations of C₃mim]BF₄ (0,100,200,300,400 and 500 mg·L^-1). The results showed that the seed germination was inhibited significantly in the level of C₃mim]BF₄ above the concentration of 400 mg·L^-1, and the rate of seedling growth declined obviously in 500 mg·L^-1. The treatment of C₃mim]BF₄ increased the levels of active oxygen($\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$、H₂O₂) and the content of malonaldehyde (MDA) and proline (Pro) in leaves of pakchoi, and gradually increased with the increase of ionic liquid concentration. C₃mim]BF₄ increased the glutathione reductase (GR) activity significantly in leaves of pakchoi. The lower concentration (≤300 mg·L^-1) of C₃mim]BF₄ could promote the formation of catalase (CAT) and ascorbate peroxidase (APX). C₃mim]BF₄ above 500 mg·L^-1 inhibited the CAT activity significantly, while treatment about 400 mg·L^-1 inhibited the APX activity significantly. C₃mim]BF₄ reduced the superoxide dismutase (SOD) activity significantly in pakchoi leaves between 100500 mg·L^-1. The expression of Cu/Zn-SOD and APX genes increased with the treatment of 400 mg·L^-1 C₃mim]BF₄ for 0~3 h, while the treatment for 13 d were significantly lower than those of the control. The expression of CAT gene was up-regulated in the treatment of C₃mim]BF₄ at 400 mg·L^-1 for 0-12 h, while the expression was down-regulated after treated for 24 h. These results provided a theoretical basis for revealing the toxicity mechanism of ionic liquid.