| 芍药茎段愈伤组织诱导的研究 |
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| 引用本文： | 刘广甫,李朝梅,李静,安湘玉,张曼. 芍药茎段愈伤组织诱导的研究[J]. 河南林业科技, 2020, 40(1): 5-8,20. DOI: 10.3969/j.issn.1003-2630.2020.01.002 |
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| 作者姓名： | 刘广甫 李朝梅 李静 安湘玉 张曼 |
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| 作者单位： | 河南省工人文化宫,河南郑州 450000;河南农业大学林学院,河南郑州 450002 |
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| 摘 要： | 以芍药品种‘粉玉奴’茎段为实验材料,1/2 MS培养基为基本培养基,探究不同浓度的6-BA以及不同光质对芍药茎段愈伤组织诱导的影响。试验结果表明,6-BA浓度为0.5mg/L时芍药茎段愈伤组织诱导情况最好;最适宜芍药茎段愈伤诱导的培养基为:1/2MS+Ca^2+(WPM)+PVP 1.0 g/L+NAA 1mg/L+LH 0.5g/L+蔗糖30g/L+琼脂7g/L+6-BA 0.5mg/L;不同的光质条件下,红光更有利于芍药进行愈伤组织诱导。
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| 关 键 词： | 芍药 茎段 愈伤诱导 光质 |
Callus Induction From Stem Segment of Paeonia lactiflora |
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| Liu Guangpu,Li Chaomei,Li Jing,An Xiangyu,Zhang Man. Callus Induction From Stem Segment of Paeonia lactiflora[J]. Journal of Henan Forestry Science and Technology, 2020, 40(1): 5-8,20. DOI: 10.3969/j.issn.1003-2630.2020.01.002 |
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| Authors: | Liu Guangpu Li Chaomei Li Jing An Xiangyu Zhang Man |
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| Affiliation: | (Colleg of Forestry,Henan Agriculture University,Zhengzhou 450002,China;Henan Workers Cultural Palace,Zhengzhou 450000,China) |
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| Abstract: | Stem of herbaceous peony(Paeonia lactiflora)‘Fen Yu Nu’was used as explants for callus induction.The effects of different concentration of plant growth regulators 6–BA and light quality on induction were investigated to establish aseptic regeneration system under 1/2MS basic medium.The results showed that the best medium for callus induction was when 6-BA was less than NAA,the best medium to induce callus was 1/2MS+Ca2+(WPM)+PVP1.0 g/L+NAA1.0 mg/L+LH 0.5g/L+sucrose30 g/L+agar7 g/L+6-BA0.5mg/L,and red lihght was more conducive to callus induction under different light quality conditions. |
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| Keywords: | Paeonia lactiflora stem callus induction light quality |
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