龙须菜细胞壁多糖对硼的吸附性能研究

为研究龙须菜(Gracilaria lemaneiformis)细胞壁多糖对硼的吸附作用机制及官能团之间的交互作用,本试验分别采用电感耦合等离子发射光谱(ICP-OES)和傅里叶变换红外光谱(FTIR)技术对其细胞壁多糖组分在吸附试验中进行硼的测定和表征。结果表明,细胞壁去琼胶后,硼吸附量减少50.13%,半纤维素去除后,硼吸附量减少21.20%,故可得纤维素吸附量占细胞壁总硼吸附量的28.67%。同时,通过细胞壁不同组分脱硼及硼胁迫后的红外光谱表征结果可知,龙须菜细胞壁及多糖在吸附硼的过程中,羟基、羧基、多糖碳链C-C为硼离子的主要结合位点,其中,琼胶、半纤维素中起主要作用的官能团为羟基、羧基,纤维素中起主要作用的官能团为多糖碳链C-C。本研究结果为进一步探究龙须菜多糖组分与硼的内在结合机制提供了基础依据。

Adsorption of Boron by Cell Wall Polysaccharide of Gracilaria lemaneiformis

In order to study the mechanism of boron adsorption by cell wall polysaccharides of Gracilaria lemaneiformis and the interaction between functional groups, the boron adsorption experiment with cell wall polysaccharides of Gracilaria lemaneiformis was determined and characterized by inductively coupled plasma optical emission Spectrometry (ICP-OES) and fourier transform infrared (FTIR) Spectrometer. The results showed that the adsorption capacity of boron decreased by 50.13% and 21.20% after the removal of agar and hemicellulose, respectively. The adsorption capacity of cellulose accounted for 28.67% of the total adsorption capacity of boron in the cell wall. The results of FTIR spectrum for different components of cell wall after boron removal and boron stress showed that hydroxyl, carboxyl and C-C in polysaccharide were the main binding sites of boron ions. The main functional groups of agar and hemicellulose were hydroxyl and carboxyl, and the main functional group of cellulose was the polysaccharide carbon chain. The results can provide a basis for the future study on the internal binding mechanism of polysaccharide and boron in Gracilaria lemaneiformis.