甜瓜VQ家族基因鉴定及白粉病菌响应分析

VQ家族基因是植物特有的一类基因,其编码蛋白主要与WRKY蛋白相互作用协同调控下游基因表达,在植物生长发育及抵御各种外界环境胁迫中起重要作用。为揭示甜瓜VQ家族基因在抗白粉病中的功能,本研究采用生物信息学方法从甜瓜基因组中鉴定到26个VQ家族基因,这些基因不均匀地分布在甜瓜11条染色体上。系统进化分析表明,该家族成员分布于不同的分支中,分别与拟南芥VQ家族蛋白不同成员聚在一起。基因结构分析表明,甜瓜VQ家族基因序列均无内含子,只有1个外显子。组织表达分析表明,1个甜瓜VQ家族基因组成性表达,23个甜瓜VQ家族基因组织特异性表达。此外,白粉病菌侵染之后,6个VQ家族基因呈现不同程度的响应。本研究结果为进一步研究甜瓜VQ家族基因在甜瓜抗白粉病中的功能奠定了理论基础。

Genome-Wide Identification of the VQ Family Genes and Response of the VQ to Powdery Mildew Infection in Melon

VQ family genes are kind of plant-specific genes, which encoding VQ motif-containing proteins that can interact with WRKY protein to regulate downstream genes and play crucial roles in the development and various stress responses in plants. In the present study, 26 VQ family genes were identified from the genome of melon by bioinformatics approach, which were randomly and unevenly distributed on 11 chromosomes. Phylogenetic analysis indicated that melon VQ proteins could be classified into seven groups, VQ in different group can play different biological function in different pathway. Gene structure analysis showed that melon VQ genes only have one exon, indicating the gene was highly conserved. Gene expression patterns analysis showed that one VQ family gene was constitutively expressed in almost all tissues and 23 VQ family genes expressed only in specific tissue. Six VQ family genes were regulated by powdery mildew infection, indicating these genes play important roles in resisting to powdery mildew disease in melon.