适合马铃薯晚疫病菌生长的培养基改良试验

由致病疫霉Phytophthora infestans引起的晚疫病是全球第一大作物病害,对马铃薯生产危害非常严重。在对该病菌的研究中确定适宜的培养基是比较关键的一环。本文采用完全随机设计,将晚疫病菌接种到黑麦与胡萝卜不同混合比例的5种培养基上,通过隔日调查的方法,比较其菌落生长直径的大小和产孢量的多少,以此作为衡量5种培养基培养效果的指标。研究结果表明:黑麦与胡萝卜的比例为1:1时,晚疫病菌的菌落生长速度最快,产孢量最多,培养效果最好。因此,可以利用这种培养基来培养马铃薯晚疫病菌,这对开展晚疫病菌的进一步研究具有一定的实际意义。

Modification of Media Suitable for Growing Phytophthora infestans

Late blight, caused by Phytophthora infestans, is most devastating disease of crops worldwide, in particular for potato. It is crucial to establish a medium suitable for growing the fungus in order to study it. In this research, five media, including extracts of rye and carrot in different combinations, were compared for colony diameter and sporangium production, which were investigated at intervals after inoculation in a complete randomized design. The data indicated that the medium with extract of rye and carrot in a ratio of 1 to 1 gave the best result, in which the fungus grew quickly and produced more sporangia. Therefore, this medium could be used for growing P. infestans and would facilitate study of the fungus.