| 五味子组织培养技术初探 |
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| 引用本文： | 李兴亮,滕世杰,才国彬. 五味子组织培养技术初探[J]. 吉林林业科技, 2008, 37(2): 7-10 |
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| 作者姓名： | 李兴亮 滕世杰 才国彬 |
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| 作者单位： | 汪清县林业局,吉林,延边,133200;吉林省林业厅,吉林,长春,130022 |
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| 摘 要： | 以五味子带芽鳞的休眠芽、不带芽鳞的休眠芽和水培的新梢茎段为外植体,接种于不同激素配比的培养基中,进行组织培养快繁技术研究。结果表明,水培的新梢茎段是诱导丛生芽的最佳外植体,在MS 6-BA1.5 mg.L-1 IBA0.2 mg.L-1、pH值5.8的初代培养基中,培养效果最为明显,分化率达到了95%。
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| 关 键 词： | 五味子 组织培养 快速繁殖 分化率 |
| 文章编号： | 1005-7129(2008)02-0007-04 |
| 修稿时间： | 2007-10-17 |
The technique of tissue culture for Schisandra chinensis |
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| LI Xing-liang,TENG Shi-jie,CAI Guo-bin. The technique of tissue culture for Schisandra chinensis[J]. Jilin Forestry Scicnce and Technology, 2008, 37(2): 7-10 |
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| Authors: | LI Xing-liang TENG Shi-jie CAI Guo-bin |
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| Abstract: | In study on the technique of tissue culture for Schisandra chinensis,the dormant bud with scale or removed or shoot rinsed then disinfected are used as explants.The culture medium modified with 30g/L sucrose 8g/L agar-agar or different concentrations growth regulator used for culture initiation.The shoot is the best explant used for axillary shoot multiplication and the best medium for initial culture is MS 6-BA1.5g/L IBA0.2mg/L at pH 5.8.The frequency of diffferentiation is 95%. |
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| Keywords: | Schisandra chinensis tissue culture rapid propagation differentiation |
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