Vehicle Effects on the <Emphasis Type="Italic">In Vitro</Emphasis> Penetration of Testosterone through Equine Skin |
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Authors: | P C Mills |
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Institution: | (1) School of Veterinary Science, University of Queensland, Brisbane, Queensland, Australia |
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Abstract: | The effects of three vehicles, phosphate-buffered saline (PBS), ethanol (50% in PBS w/w) and propylene glycol (50% in PBS
w/w) on in vitro transdermal penetration of testosterone was investigated in the horse. Skin was harvested from the thorax of five Thoroughbred
horses after euthanasia and stored at −20°C until required. The skin was then defrosted and placed into Franz-type diffusion cells, which were maintained at approximately
32°C by a water bath. Saturated solutions of testosterone, containing trace amounts of radiolabelled 14C]testosterone, in each vehicle were applied to the outer (stratum corneum) surface of each skin sample and aliquots of receptor
fluid were collected at 0, 2, 4, 8, 16, 20, 22 and 24 h and analysed for testosterone by scintillation counting. The maximum
flux (J
max) of testosterone was significantly higher for all sites when testosterone was dissolved in a vehicle containing 50% ethanol
or 50% propylene glycol, compared to PBS. In contrast, higher residues of testosterone were found remaining within the skin
when PBS was used as a vehicle. This study shows that variability in clinical response to testosterone could be expected with
formulation design. |
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Keywords: | horse testosterone Thoroughbred topical transdermal |
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