不同培养基对辣椒花药培养的影响

以灯笼椒、泡椒、线椒3种类型21个辣椒品种为试材,研究NTH、MS、B5培养基和在NTH、MS、B5培养基中分别添加3g/L活性炭+0.5mg/LNAA+1.0mg/LKT+0.1mg/L6-BA+5mg/LAgNO3的培养基NTH1、MS1、B51对辣椒花药培养效果的影响.结果表明,在供试品种中能诱导出胚状体的品种占71.43%,胚状体诱导率为0.41%～2.69%;按类型统计,分别有83.33%的灯笼椒、69.23%的泡椒、50.00%的线椒能诱导出胚状体;愈伤组织诱导率和胚状体诱导率的平均值从大到小依次为MS1、NTH1、B51、MS、NTH、B5,其中,MS1和NTH1差异不显著,两者极显著高于B51;NTH1、MS1、B51培养基处理对减轻褐化具有明显效果,并能显著提高愈伤组织和胚状体诱导率;愈伤组织诱导率和胚状体诱导率MS与NTH差异不显著,MS和NTH与B5培养基差异均达极显著水平

Effects of culture media on anther culture of chili pepper (Capsicum annuum L.)

The effects on anther culture of six culture media including NTH, MS, B5 and NTH1, MS1, B51 (i.e. the modified NTH, MS or B5 supplemented with 3g/L activated charcoal, 0.5 mg/L NAA, 1.0 mg/L KT+ 0.1 mg/L 6-BA +5 mg/L AgNO3)were studied using 21 capsicum varieties belonging to the three fruit shape types (sent pepper, bell pepper and hot pepper) as the materials. The results showed that 71.43% of the varieties used produced embryoids with induction frequency of 0.41%~2.69%. Among the fruit shape types, 83.33% of the sent varieties, 69.23% of the bell varieties and 50.00% of the hot varieties produced embryoids. Addition of 5 mg/L AgNO3 into the media have significantly reduced browning. The order of the mean values of both the induction percentage of calli and that of embryoids in anther culture by six media was MS1,NTH1,B51,MS,NTH,B5. Although MS1 and NTH1 did not show the difference, they were significantly superior to B5. NTH1, MS1 and B51 significantly reduced browning and improved percentage of induction of both calli and embryoid. Although there was not any significant difference in the percentage of induction between MS and NTH, the percentage of induction on MS or NTH was significantly higher than that on B5.