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| 相思基因组DNA提取及其RAPD初步分析 | |
| 引用本文： | 张卫华,黄烈健,陈祖旭,徐大平,张守攻,张方秋,龚峥.相思基因组DNA提取及其RAPD初步分析[J].广东林业科技,2007,23(4):1-5. |
| 作者姓名： | 张卫华 黄烈健 陈祖旭 徐大平 张守攻 张方秋 龚峥 |
| 作者单位： | 1. 广东省林业科学研究院,广东,广州,510520;中国林业科学研究院热带林业研究所 2. 中国林业科学研究院热带林业研究所 3. 中国林业科学研究院林业研究所 4. 广东省林业科学研究院,广东,广州,510520 |
| 基金项目： | “十五”国家科技攻关项目(2002BA515B0202)，“948”引进国际创新项目(2001-C2) |
| 摘 要： | 以马占相思、大叶相思、厚荚相思幼苗的叶片为材料,采用SDS法、改良SDS法和改良CTAB法进行了基因组DNA的提取并对DNA提取方法作了比较,结果表明改良CTAB法提取效果最好。同时利用改良CTAB法提取出的DNA为模板进行了RAPD分析,鉴别了不同树种之间的多态性。其中有11个引物共扩增出117条谱带,树种间多态性比率为54.7%,遗传多态性明显高于种内。 |
| 关 键 词： | 相思 SDS CTAB DNA RAPD 遗传多样性 |
| 文章编号： | 1006-4427（2007）04-0001-05 |
Extraction of Genomic DNA and RAPD Analysis on Acacia |
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| Zhang Weihua,Huang Liejian,Chen Zuxu,Xu Daping,Zhang Shougong,Zhang Fangqiu,Gong Zheng.Extraction of Genomic DNA and RAPD Analysis on Acacia[J].Forestry Science and Technology of Guangdong Province,2007,23(4):1-5. | |
| Authors: | Zhang Weihua Huang Liejian Chen Zuxu Xu Daping Zhang Shougong Zhang Fangqiu Gong Zheng |
| Institution: | 1. Guangdong Forest Research Institute, Guangdong Guangzhou, 510520; 2. China Research Institute of Tropical Forestry, CAF; 3. Research Institute of Forestry, CAF |
| Abstract: | The methods of SDS,modified SDS and modified CTAB were adopted to extract genomic DNA from leaf tissues of Acacia mangium,A.auriculiformis and A.crasscarpa.The results showed that modified CTAB method was the best method.In addition,the paper analyzed the genetic diversity of Acacia using RAPD,based on DNA which was extracted by modified CTAB method was employed as template.A total of 117 sites were detected by 11 perimers,which the proportion of polymorphic loci was 54.70%. |
| Keywords: | Acacia SDS CTAB DNA RAPD genetic diversity |
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