| 黄瓜-酸黄瓜渐渗系霜霉病抗性及感病前后几种酶活性的变化 |
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| 引用本文： | 曹清河,陈劲枫,李英,刁卫平,万红建. 黄瓜-酸黄瓜渐渗系霜霉病抗性及感病前后几种酶活性的变化[J]. 植物病理学报, 2007, 37(4): 433-437 |
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| 作者姓名： | 曹清河 陈劲枫 李英 刁卫平 万红建 |
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| 作者单位： | 作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院, 南京 210095 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金;江苏省高校高新技术产业发展项目;国家科技支撑计划 |
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| 摘 要： | Using resistant and susceptible cultivars as controls, the resistant evaluation to Pseudoperonospora cubensis was carried out in 12 introgression lines from wide cross between cucumber(Cucumis sativus L.) and sour cucumber(Cucumis hystrix Chakr.). Three enzyme activities including POD, SOD and PAL were analyzed before and 7 d after inoculation, and the POD isozyme was detected by polyacrylamide electrophoresis. The inoculation results showed that, of the 12 accessions, 3 were identified as high resistant, 5 were moderately resistant and 4 were moderately susceptible to downy mildew. The enzyme activities of POD, SOD and PAL were greatly increased in resistant accessions after inoculation. PAL enzyme activities showed close correlation with disease rating before or after inoculation, which implicated that PAL enzyme activity might be used to estimate the resistance to downy mildew. POD isozyme electrophoresis showed that the number and intensity for the bands of resistant lines were significantly increased more than those of susceptible lines after inoculation.
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| 关 键 词： | 黄瓜-酸黄瓜渐渗系 霜霉病 抗性 酶活性 霜霉病菌 |
| 文章编号： | 0412-0914（2007）04-0433-05 |
| 修稿时间： | 2006-01-23 |
Downy mildew resistance and changes of some enzyme activities before and after infection with Pseudoperonospora cubensis in cucumber-sour cucumber introgression lines |
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| CAO Qing-he,CHEN Jin-feng,LI Ying,DIAO Wei-ping,WAN Hong-jian. Downy mildew resistance and changes of some enzyme activities before and after infection with Pseudoperonospora cubensis in cucumber-sour cucumber introgression lines[J]. Acta Phytopathologica Sinica, 2007, 37(4): 433-437 |
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| Authors: | CAO Qing-he CHEN Jin-feng LI Ying DIAO Wei-ping WAN Hong-jian |
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| Affiliation: | State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Using resistant and susceptible cultivars as controls, the resistant evaluation to Pseudoperonospora cubensis was carried out in 12 introgression lines from wide cross between cucumber(Cucumis sativus L.) and sour cucumber(Cucumis hystrix Chakr.). Three enzyme activities including POD, SOD and PAL were analyzed before and 7 d after inoculation, and the POD isozyme was detected by polyacrylamide electrophoresis. The inoculation results showed that, of the 12 accessions, 3 were identified as high resistant, 5 were moderately resistant and 4 were moderately susceptible to downy mildew. The enzyme activities of POD, SOD and PAL were greatly increased in resistant accessions after inoculation. PAL enzyme activities showed close correlation with disease rating before or after inoculation, which implicated that PAL enzyme activity might be used to estimate the resistance to downy mildew. POD isozyme electrophoresis showed that the number and intensity for the bands of resistant lines were significantly increased more than those of susceptible lines after inoculation. |
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| Keywords: | introgression lines downy mildew enzyme activity POD isozyme electrophoresis |
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