| 应用基因芯片检测烟草脆裂病毒 |
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| 引用本文： | 马新颖,汪琳,任鲁风,邓丛良,高文娜,汪万春,周琦.应用基因芯片检测烟草脆裂病毒[J].植物病理学报,2007,37(4):352-355. |
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| 作者姓名： | 马新颖 汪琳 任鲁风 邓丛良 高文娜 汪万春 周琦 |
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| 作者单位： | 1 北京出入境检验检疫局, 北京 100026;2 本元正阳基因技术有限公司, 北京 100176;3 中国科学院研究生院, 北京 100049 |
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| 基金项目： | 国家食品安全重大专项项目;国家高技术研究发展计划(863计划) |
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| 摘 要： | Total RNA in tulips was extracted by Trizol method. Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant. The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP. The probes of plant virus, 18S rRNA gene and comparisons were designed and immobilized on chips. Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software. Tobacco rattle virus was detected from tulips which were imported from Holand. The accuracy and sensitivity of the plant virus gene chip were proved.
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| 关 键 词： | 基因芯片 检测 烟草脆裂病毒 有害生物 检疫 |
| 文章编号： | 0412-0914（2007）04-3524-04 |
| 修稿时间： | 2006-07-19 |
Gene chip detecting for Tobacco rattle virus |
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| MA Xin-ying,WANG Lin,REN Lu-feng,DENG Cong-liang,GAO Wen-na,WANG Wan-chun,ZHOU Qi.Gene chip detecting for Tobacco rattle virus[J].Acta Phytopathologica Sinica,2007,37(4):352-355. |
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| Authors: | MA Xin-ying WANG Lin REN Lu-feng DENG Cong-liang GAO Wen-na WANG Wan-chun ZHOU Qi |
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| Institution: | 1 Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China;2 Benyuan Zhengyang Gene Technology Joint-Stock Company, Beijing 100176, China;3 Graduate of University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Total RNA in tulips was extracted by Trizol method.Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant.The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP.The probes of plant virus,18S rRNA gene and comparisons were designed and immobilized on chips.Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software.Tobacco rattle virus was detected from tulips which were imported from Holand.The accuracy and sensitivity of the plant virus gene chip were proved. |
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| Keywords: | gene chip hybridization phytosanitory Tobacco rattle virus |
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