| 马铃薯疮痂病菌致病相关基因的克隆及表达 |
| |
| 引用本文： | 赵伟全,刘大群,杨文香,张汀.马铃薯疮痂病菌致病相关基因的克隆及表达[J].植物病理学报,2007,37(4):442-445. |
| |
| 作者姓名： | 赵伟全 刘大群 杨文香 张汀 |
| |
| 作者单位： | 河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 保定 071001 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金;河北农业大学校科研和教改项目 |
| |
| 摘 要： | A pathogenic-related gene nec1 was cloned in Streptomyces scabies CPS-1, potato scab strain. Analysis results showed that the length of open reading frame(ORF) for nec1 gene was 666 bp, and the GC content was 54.2%. Sequence alignment indicated that a 650 bp up-stream sequence shared 91% similarity with IS 256 family transposase nucleotide sequences by BLASTn searches against GenBank. The segments obtained by PCR amplification were digested by enzymes SphⅠand SacⅠ, and linked to the expression vector pIJ702. The recombinants were transformed into nonpathogenicity strain Streptomyces lividans 66 TK24. Bioas-say results suggested that the transformants possessed the same symptoms as pathogenic strain on potato tuber slices and radish seedlings, which implied that nec1 gene was associated with the pathogenicity of S. scabies CPS-1.
|
| 关 键 词： | 马铃薯 疮痂病菌 致病相关基因 克隆 表达 疮痂病 |
| 文章编号： | 0412-0914（2007）04-0442-04 |
| 修稿时间： | 2006-04-13 |
Cloning and expression of a pathogenic-related gene in Streptomyces scabies CPS-1 |
| |
| ZHAO Wei-quan,LIU Da-qun,YANG Wen-xiang,ZHANG Ting.Cloning and expression of a pathogenic-related gene in Streptomyces scabies CPS-1[J].Acta Phytopathologica Sinica,2007,37(4):442-445. |
| |
| Authors: | ZHAO Wei-quan LIU Da-qun YANG Wen-xiang ZHANG Ting |
| |
| Institution: | College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China |
| |
| Abstract: | A pathogenic-related gene nec1 was cloned in Streptomyces scabies CPS-1, potato scab strain. Analysis results showed that the length of open reading frame(ORF) for nec1 gene was 666 bp, and the GC content was 54.2%. Sequence alignment indicated that a 650 bp up-stream sequence shared 91% similarity with IS 256 family transposase nucleotide sequences by BLASTn searches against GenBank. The segments obtained by PCR amplification were digested by enzymes SphⅠand SacⅠ, and linked to the expression vector pIJ702. The recombinants were transformed into nonpathogenicity strain Streptomyces lividans 66 TK24. Bioas-say results suggested that the transformants possessed the same symptoms as pathogenic strain on potato tuber slices and radish seedlings, which implied that nec1 gene was associated with the pathogenicity of S. scabies CPS-1. |
| |
| Keywords: | potato scab nec1 gene cloning transformant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
