| 黄瓜霜霉病菌保存方法 |
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| 引用本文： | 张艳菊,秦智伟,周秀艳,徐生军,苏亚非,蔡宁.黄瓜霜霉病菌保存方法[J].植物病理学报,2007,37(4):438-441. |
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| 作者姓名： | 张艳菊 秦智伟 周秀艳 徐生军 苏亚非 蔡宁 |
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| 作者单位： | 东北农业大学, 哈尔滨 150030 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目;国家高技术研究发展计划(863计划) |
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| 摘 要： | Pseudoperonospora cubensis(Berk. & M. A. Curtis) Rostovzev, the causal agent of cucumber downy mildew, is an obligate parasitic fungus. Up to now it can not be preserved on culture medium. In this study, the sporangia of P. cubensis were preserved in protective substances of 10% dimethyl sulfoxide plus 5% skim milk, 10% dimethyl sulfoxide plus 10% skim milk, sterilized water and leaves in vitro, and stored at -20℃, -70℃, and preliminary freezing at -20℃ for 24 h prior to -70℃ respectively. The results showed that the sporangia preserved in 10% dimethyl sulfoxide plus 5% skim milk, preliminary freezing at -20℃ for 24 h prior to -70℃ were still highly pathogenic after 12 months preservation. The percentage of germination of sporangia, incidence and disease index were 46%, 50.0% and 40.0 respectively 9 d after inoculation. This method solved the problem of P. cubensis preservation.
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| 关 键 词： | 黄瓜 霜霉病菌 保存方法 霜霉病 |
| 文章编号： | 0412-0914（2007）04-0438-04 |
| 修稿时间： | 2006-06-19 |
The preservation of Pseudoperonospora cubensis |
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| ZHANG Yan-ju,QIN Zhi-wei,ZHOU Xiu-yan,XU Sheng-jun,SU Ya-fei,CAI Ning.The preservation of Pseudoperonospora cubensis[J].Acta Phytopathologica Sinica,2007,37(4):438-441. |
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| Authors: | ZHANG Yan-ju QIN Zhi-wei ZHOU Xiu-yan XU Sheng-jun SU Ya-fei CAI Ning |
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| Institution: | Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Pseudoperonospora cubensis(Berk. & M. A. Curtis) Rostovzev, the causal agent of cucumber downy mildew, is an obligate parasitic fungus. Up to now it can not be preserved on culture medium. In this study, the sporangia of P. cubensis were preserved in protective substances of 10% dimethyl sulfoxide plus 5% skim milk, 10% dimethyl sulfoxide plus 10% skim milk, sterilized water and leaves in vitro, and stored at -20℃, -70℃, and preliminary freezing at -20℃ for 24 h prior to -70℃ respectively. The results showed that the sporangia preserved in 10% dimethyl sulfoxide plus 5% skim milk, preliminary freezing at -20℃ for 24 h prior to -70℃ were still highly pathogenic after 12 months preservation. The percentage of germination of sporangia, incidence and disease index were 46%, 50.0% and 40.0 respectively 9 d after inoculation. This method solved the problem of P. cubensis preservation. |
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| Keywords: | Pseudoperonospora cubensis preservation sporangia dimethyl sulfoxide and skim milk pathogenicity |
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