| 小麦蓝矮病植原体转运蛋白secY基因片段序列分析 |
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| 引用本文： | 张荣,崔晓艳,孙广宇,康振生. 小麦蓝矮病植原体转运蛋白secY基因片段序列分析[J]. 植物病理学报, 2007, 37(4): 446-448 |
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| 作者姓名： | 张荣 崔晓艳 孙广宇 康振生 |
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| 作者单位： | 西北农林科技大学植物保护学院, 杨凌 712100 |
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| 基金项目： | 国家自然科学基金;教育部长江学者和创新团队发展计划创新团队项目;陕西省自然科学基金 |
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| 摘 要： | Wheat blue dwarf(WBD) is a disease caused by phytoplasma and only reported from China. A fragment about 1.3 kb in protein translocation gene, secY was amplified by PCR from the total DNA of di-seased wheat sample with primer pair secYF/secYR, which was designed based on secY gene sequence of known 16SrI group members. Nucleotide acid sequence analysis of amplified fragment indicated that the length was 1 240 bp. A phylogenetic tree based on secY gene sequences was constructed and showed that wheat blue dwarf phytoplasma was clustered into the Candidatus Phytoplasma asteris, subgroup 16SrI-C. Wheat blue dwarf phytoplasma showed high homology with clover phyllody phytoplasma strains based on sequence comparison and phylogenetic analysis.
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| 关 键 词： | 小麦 蓝矮病 植原体 转运蛋白secY基因片段 序列分析 |
| 文章编号： | 0412-0914（2007）04-0446-03 |
| 收稿时间： | 2006-05-19 |
| 修稿时间： | 2006-05-19 |
SecY gene sequence analysis of phytoplasma associated with wheat blue dwarf |
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| ZHANG Rong,CUI Xiao-yan,SUN Guang-yu,KANG Zhen-sheng. SecY gene sequence analysis of phytoplasma associated with wheat blue dwarf[J]. Acta Phytopathologica Sinica, 2007, 37(4): 446-448 |
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| Authors: | ZHANG Rong CUI Xiao-yan SUN Guang-yu KANG Zhen-sheng |
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| Affiliation: | College of Plant Protection, Northwest A & F University, Yangling 712100, China |
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| Abstract: | Wheat blue dwarf(WBD)is a disease caused by phytoplasma and only reported from China.A fragment about 1.3 kb in protein translocation gene,secY was amplified by PCR from the total DNA of di-seased wheat sample with primer pair secYF/secYR,which was designed based on secY gene sequence of known 16SrI group members.Nucleotide acid sequence analysis of amplified fragment indicated that the length was 1 240 bp.A phylogenetic tree based on secY gene sequences was constructed and showed that wheat blue dwarf phytoplasma was clustered into the Candidatus Phytoplasma asteris,subgroup 16SrI-C.Wheat blue dwarf phytoplasma showed high homology with clover phyllody phytoplasma strains based on sequence comparison and phylogenetic analysis. |
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| Keywords: | phylogenetics clover phyllody phytoplasma Candidatus Phytoplasma asteris classification |
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