棉铃虫核多角体病毒的生产方法及其在生物防治中的应用

本文是报道棉铃虫Heliothis armigera核多角体病毒(NPV)的生产方法及其在棉田应用的试验研究结果。NPV用棉铃虫幼虫生产。从虫卵接种开始,用人工饲料饲养棉铃虫幼虫,到四龄用病毒感染,六天后收集病死虫,病毒致死率80～85%,单虫含量4～6×10~9PIB。由棉饼粉、粗棉油等配制成诱饵加低剂量西维因作病毒增效剂与NPV混用,显著提高病毒的防治效果。四年田间试验表明,NPV杀虫剂防治棉铃虫的效果相当于或优于常用高效化学农药。1979年开展了大面积飞机微量喷雾PVN杀虫剂的试验,也取得了比较好的防治效果。

THE PRODUCTION AND APPLICATION OF THE NUCLEAR POLYHEDROSIS VIRUS OF HELIOTHIS ARMIGERA ARMIGERA(HUBNER)IN BIOLOGICAL CONTROL

The present paper reports a production technique for the nuclear polyhedrosis virus (NPV) of H. armigera and its effectiveness against the population of H. armigera in cotton fields.The larvae of H. armigera were used for NPV production.Subsequent to the egg inoculation, the larvae were individually reared on artificial diet in small bottles. Preoral infection of the fourth instar larvae was performed by contamination on diet surface. Infected larvae were then collected after 6 days. Their mortality was found to be 80-85% and individual larva produced 4-6×10⁹ PIB.Combination of NPV with bait, formulated from cottonseed cake powder and crude cottonseed oil, etc, and low dosage of carbaryl enhanced significantly the controling effect against H. armigera. Field tests in 1976-1979 for the control of H. armigera showed that the effectiveness of the NPV insecticide was equivalent or superior to that of standard chemical insecticides. Large scale field tests on cotton with low volume spray of the NPV insecticide from a plane were conducted in 1979 and effective control of H. armigera had been obtained.