Preparation and evaluation of antioxidant capacity of Jackfruit (Artocarpus heterophyllus Lam.) wine and its protective role against radiation induced DNA damage |
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Authors: | Umesh B JagtapShailesh R Waghmare Vinayak H LokhandePenna Suprasanna Vishwas A Bapat |
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Institution: | a Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416 004, (MS) India b Department of Microbiology, Shivaji University, Vidyanagar, Kolhapur 416 004, (MS) India c Department of Botany, University of Pune, Pune 411 007, (MS) India d Functional Plant Biology section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic research centre, Trombay, Mumbai 400 085, (MS) India |
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Abstract: | Jackfruit is an underutilized edible fruit in the tropics and subtropics. The aim of this study was to produce wine from jackfruit pulp and to evaluate the total phenolic and flavonoid contents and antioxidant properties of the wine. The ability of scavenging free radicals was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant assay (FRAP), N, N-dimethyl-p-phenylendiamine (DMPD) and nitric oxide (NO) scavenging assays. Experimental results indicated that jackfruit wine was effective in DPPH radical scavenging (69.44 ± 0.34%), FRAP (0.358 optical density value, O.D.), DMPD (78.45 ± 0.05%) and NO (62.46 ± 0.45%) capacity. By the analysis of the high performance liquid chromatography coupled to diode array detector (HPLC-DAD), two phenolic compounds namely gallic acid and protocatechuic acid were identified. The jackfruit wine was also able to protect H2O2 + UV radiation and γ-radiation (100 Gy) induced DNA damage in pBR322 plasmid DNA. The antioxidant and DNA damage protecting properties of jackfruit wine confirmed health benefits when consumed and could become a valuable source of antioxidant rich neutraceuticals. Additionally, the wine could be a commercially valuable by-product for the jackfruit growers. |
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Keywords: | DMPD N N-dimethyl-p-phenylendiamine DPPH 2 2-diphenyl-1-picrylhydrazyl FRAP ferric reducing antioxidant assay HPLC high pressure liquid chromatography JFW jackfruit wine NO nitric oxide RSC radical scavenging capacity SNP sodium nitroprusside |
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