| 贴壁和悬浮状态293T细胞慢病毒包装效果的比较研究 |
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| 引用本文： | 孙克宁,林峰,朱化彬,郝海生,杜卫华,赵学明,刘岩,秦彤,王栋. 贴壁和悬浮状态293T细胞慢病毒包装效果的比较研究[J]. 河南农业大学学报, 2011, 0(6): 668-671,687 |
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| 作者姓名： | 孙克宁 林峰 朱化彬 郝海生 杜卫华 赵学明 刘岩 秦彤 王栋 |
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| 作者单位： | 河南农业大学牧医工程学院;中国农业科学院北京畜牧兽医研究所; |
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| 基金项目： | 农业部转基因重大专项(2009ZX08010-024B) |
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| 摘 要： | 为提高慢病毒包装效率,比较了贴壁和悬浮293T细胞的磷酸钙法慢病毒的包装效果.结果表明,利用悬浮细胞获得的病毒滴度较贴壁细胞高近3.5倍,同时这种方法具有很好的重复性,且悬浮细胞可直接用于病毒包装,节省了24 h的细胞培养时间.
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| 关 键 词： | 慢病毒 包装 转染 |
Comparison of effects on lentivirus packaging between adherent and suspended 293T cells |
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| SUN Ke-ning,LIN Feng,ZHU Hua-bin,HAO Hai-sheng,DU Wei-hua,ZHAO Xue-ming,LIU Yan,QIN Tong,WANG Dong. Comparison of effects on lentivirus packaging between adherent and suspended 293T cells[J]. Journal of Henan Agricultural University, 2011, 0(6): 668-671,687 |
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| Authors: | SUN Ke-ning LIN Feng ZHU Hua-bin HAO Hai-sheng DU Wei-hua ZHAO Xue-ming LIU Yan QIN Tong WANG Dong |
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| Affiliation: | SUN Ke-ning1,LIN Feng1,ZHU Hua-bin2,HAO Hai-sheng2,DU Wei-hua2,ZHAO Xue-ming2,LIU Yan2,QIN Tong2,WANG Dong2(1.College of Animal Science and Vetrinary Medicine,Henan Agricultural University,Zhengzhou 450002,China,2.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | To improve the efficiency of lentivirus packaging,the influences of state of cell growth and existence on virus packaging were studied;the effects of adherent and suspended 293T cells on packaging lentivirus were compared using calcium phosphate method.The results show that the titer of lentivirus packaged with suspension cells was nearly 3.5 folds higher than that packaged with adherent cells.Moreover,two advantages,higher repeatability and less cell culture time which was at least 24 h shorter,of lentivir... |
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| Keywords: | lentivirus packaging transfection |
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