类番茄茄与番茄属间有性杂交的研究

用类番茄茄与番茄属的 9个种进行有性杂交 ,通过人工离体胚培养获得栽培番茄‘粤农二号’×类番茄茄的F1植株。杂种近于不育。用栽培番茄、类番茄茄、野生番茄及(栽培番茄×野生番茄 )与之回交及杂交 ,获得与栽培番茄的回交子一代及与秘鲁番茄、多腺番茄、潘那利番茄、粤农二号×秘鲁番茄F1的杂种 ,但它们的能育性很低。用栽培番茄、野生番茄与它们再回交及杂交 ,未能得到与栽培番茄的二次回交子一代 ,只获得 (粤农二号×类番茄茄 )× (粤农二号×秘鲁番茄 )的F1与秘鲁番茄、多腺番茄的复合杂种。人工苗期接种黄瓜花叶病毒 (CMV)结果表明 ,类番茄茄及其与番茄属的远缘杂种对CMV有较强的抗性。

Studies on Generic Cross between Solanum lycopersicoides and Lyco-persicon sp.

AIM:To explore the effect of carbon monoxide (CO) on hypoxic proliferation of rat pulmonary arterial smooth muscle cell(PASMC). METHODS:Rats were randomly divided into four groups, including: (1)N:Normoxia group (21% O₂); (2)H:Hypoxia group (1% O₂); (3)CO+H:CO+hypoxia group (3% CO+1% O₂),tested by immunocytochemical analysis,³H]-TdR incorporation and flow cytometry (FCM). RESULTS: (1)Flow cytometric DNA analysis revealed that hypoxia induced significantly enhancement of G 2/M phase and decreased G₀/G₁ phase of PASMC (P <0.01).The quantities of G₂/M phase or G₀/G₁ phase in CO+H was about equal to that in N. (2) Immunocytochemical reaction of proliferating cell nuclear antigen (PCNA) in H of PASMC was more stronger than that in N or CO+H. The reaction of PASMC in CO+H was similar to that in N. (3)³H]-TdR incorporation was increased gradually at the end of 12 h in hypoxia and then significanlty promoted at the end of 24 h in hypoxia.But ³H]-TdR incorporation in CO+H was not much enhancedt. (4)α-actin and muscle fiber was decreased in H, but wasn’t changed in CO+H. CONCLUSION: Hypoxia may stimulate PASMC and promote proliferation of PASMC indirectly, and low density(3%) carbon monoxide can partly offset the action of hypoxia effectively.