鹿药组织培养的研究

为保护鹿药这种野生植物资源，以生长旺盛的鹿药根茎为材料，进行愈伤组织诱导、愈伤组织分化、试管苗的生根、扦插和移植的研究，成功地建立起壳药根茎的再生体系。结果证明：MS＋ZT0．2mg/L＋CH20mg／L＋2，4-D1．5mg／L（或2．0mg／L）是鹿药根茎愈伤组织诱导培养的理想培养基；MS＋AgNO3 0．2mg／L＋BA0．2mg／L＋KT0．3mg／L＋NAA0．1mg／L是鹿药愈伤组织分化培养的理想培养基；1／2MS＋BA0．4mg／L＋NAA0．1mg／L是鹿药愈伤组织分化不定芽继代培养和增殖培养的理想培养基；1／3MS＋NAA0．1mg／L＋IAA0．4mg／L是鹿药生根培养和生根继代培养的理想培养基；移植到山坡上试管苗生长旺盛。

Study of Tissue Culture of Smilacina laponica

For the protection of wild resources, the exptants from strong growth Smilacina laponica stem segment were cultured to in- vestigate the effects of different external hormones on induction and differentiation of callus, formation of adventitious roots, cutting and transplantation. The asexual line of Smilacina laponica was MS＋ZT established successfully. The results show that the ideal medium for induction and differentiation of callus were MS＋ZT0.2mg/L＋CH20mg/L＋2, 4-D1.5mg/L（or 2.0mg/L）and MS＋AgNO30.2mg/L＋ BA0.2mg/L＋KT0.3mg/L＋NAA0.1mg/L respectively, the ideal differentiation and multiplication medium for adventitious buds was 1/ 2MS＋BA0.4mg/L＋NAA0.1mg/L, the ideal medium for rooting was 1/3MS＋NAA0.1mg/L＋IAA0.4mg/L. The tube seedlings transplanted to the hill grew orderly and flourishly.